Probing Genomic Aspects of the Multi-Host Pathogen Clostridium perfringens Reveals Significant Pangenome Diversity, and a Diverse Array of Virulence Factors.

Frontiers in Microbiology
Raymond KiuLindsay J Hall

Abstract

Clostridium perfringens is an important cause of animal and human infections, however information about the genetic makeup of this pathogenic bacterium is currently limited. In this study, we sought to understand and characterise the genomic variation, pangenomic diversity, and key virulence traits of 56 C. perfringens strains which included 51 public, and 5 newly sequenced and annotated genomes using Whole Genome Sequencing. Our investigation revealed that C. perfringens has an "open" pangenome comprising 11667 genes and 12.6% of core genes, identified as the most divergent single-species Gram-positive bacterial pangenome currently reported. Our computational analyses also defined C. perfringens phylogeny (16S rRNA gene) in relation to some 25 Clostridium species, with C. baratii and C. sardiniense determined to be the closest relatives. Profiling virulence-associated factors confirmed presence of well-characterised C. perfringens-associated exotoxins genes including α-toxin (plc), enterotoxin (cpe), and Perfringolysin O (pfo or pfoA), although interestingly there did not appear to be a close correlation with encoded toxin type and disease phenotype. Furthermore, genomic analysis indicated significant horizontal gene transfer ...Continue Reading

References

Jul 1, 1976·Canadian Journal of Microbiology·D E Mahony, T I Moore
Nov 11, 1978·Lancet·A M KosloskeH Hoffman
Apr 1, 1992·International Journal of Systematic Bacteriology·B CanardS T Cole
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C R WoeseM L Wheelis
Feb 1, 1988·Japanese Journal of Medical Science & Biology·T MasakiS Nakamura
Nov 1, 1967·Journal of Bacteriology·G Paquette, V Fredette
Oct 1, 1994·International Journal of Systematic Bacteriology·M D CollinsJ A Farrow
Jan 13, 1998·Gene·M GibertC Jolivet-Renaud
Jan 19, 1999·Annual Review of Microbiology·J I Rood
Apr 22, 1999·Trends in Microbiology·L PetitM R Popoff
Feb 11, 2000·The Journal of Infectious Diseases·X ZhangD W Acheson
Jan 17, 2002·Cell·Harald Brüssow, Roger W Hendrix
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tohru ShimizuHideo Hayashi
May 31, 2002·Journal of Clinical Microbiology·Sydney M HarveyDavid E Dassey
Aug 13, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Dennis L Stevens, Amy E Bryant
Mar 1, 1953·The Journal of Hygiene·B C HOBBSJ C CRUICKSHANK
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
May 12, 2004·Journal of Molecular Biology·Nicholas ThomsonGordon Dougan
Jun 25, 2004·Nucleic Acids Research·Ji QiBailin Hao
Jan 28, 2005·Journal of Medical Microbiology·Shan GohThomas V Riley
Jan 23, 2009·Veterinary Microbiology·Alphons J A M van AstenAndrea Gröne
Oct 27, 2009·Nucleic Acids Research·Lewis Y GeerStephen H Bryant
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Dec 15, 2010·Avian Pathology : Journal of the W.V.P.A·Jessica BradyJuan C Rodriguez-Lecompte
Jan 5, 2011·Emerging Infectious Diseases·Elaine ScallanPatricia M Griffin
Aug 10, 2011·BMC Genomics·Nabil-Fareed AlikhanScott A Beatson

❮ Previous
Next ❯

Citations

Aug 8, 2018·Emerging Microbes & Infections·Raymond Kiu, Lindsay J Hall
May 28, 2019·Molecular Genetics and Genomics : MGG·Jinzhao LongGuangcai Duan
Jan 21, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Horst PosthausJulia Bruggisser
Feb 23, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Iman Mehdizadeh GohariJohn F Prescott
Dec 25, 2019·Journal of Animal Physiology and Animal Nutrition·Chongli XuChongbo Xu
Aug 25, 2020·Journal of Water and Health·Johannes Cornelius Jacobus FourieRasheed Adeleke
May 7, 2019·Frontiers in Microbiology·Abakabir Mahamat AbdelrahimOlivier Firmesse
Aug 16, 2019·Toxins·Balgabay MaikanovMagdalena Grabczak
Oct 27, 2020·Genome Génome / Conseil National De Recherches Canada·Wei ZouJiangang Yang
Apr 13, 2021·Virulence·Iman Mehdizadeh GohariBruce A McClane
May 18, 2021·Frontiers in Cellular and Infection Microbiology·Lore Van DammeEvy Goossens
Jul 28, 2019·Microbiology Spectrum·Masahiro NagahamaJulian I Rood

❮ Previous
Next ❯

Datasets Mentioned

BETA
JGS1495

Methods Mentioned

BETA
PCR
Whole Genome Sequencing
Assay
MDS

Software Mentioned

SMRTpipe
Webtool iTOL
MAFFT
HMMER
in
MinCED
house
PhyML
BAsic Rapid Ribosomal RNA Predictor ( Barrnap )
cgMLST

Related Concepts

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPRi Screens for Antibiotics

The CRISPR-Cas system is a gene editing technique that can be used for high-throughput genome-wide screens to identify modes of actions of novel antibiotics. Here is the latest research.