PMID: 8938731Jan 1, 1996Paper

Probing molecular identity of native single potassium channels by overexpression of dominant negative subunits

Neuropharmacology
A B RiberaR S Taylor

Abstract

Overexpression of dominant negative subunits previously has been shown to affect the whole cell delayed-rectifier potassium current (Ikv) in Xenopus spinal neurons. Here, we show that effects of overexpression of wild-type and dominant negative Kv1 channels are evident at the single channel level. The goal of these studies was to match molecular species of Kv subunits to specific, functionally identified single voltage-dependent potassium channels. In a heterologous system (the Xenopus oocyte), co-expression of wild-type and dominant negative mutant Kv1.1 subunits results in loss of active channels rather than channels of altered conductance. However, in situ overexpression studies are difficult to interpret due to the diversity in the control population of channels. Therefore, identification of endogenous channel populations containing Kv1 subunits is limited. Future work will reduce the endogenous diversity of potassium channels by study of single channels in identified subtypes of neurons.

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Citations

Feb 18, 1998·Current Opinion in Neurobiology·N Dale, F Kuenzi
Jul 13, 2001·Journal of Molecular and Cellular Cardiology·D J PountneyW A Coetzee
Feb 4, 2003·The Journal of Physiology·Patrick E MacDonaldMichael B Wheeler
May 17, 2012·Mechanisms of Development·J C HockingS McFarlane
Apr 17, 1999·The Journal of Biological Chemistry·R MathurG Koren
Jul 15, 2000·The Journal of Biological Chemistry·L N Manganas, J S Trimmer
Jul 21, 2001·Molecular Endocrinology·P E MacDonaldM B Wheeler

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