Probing phosphatidylinositolphosphates and adenosinenucleotides on talin nucleated actin polymerization

FEBS Letters
G IsenbergW H Goldmann

Abstract

We have investigated the binding of PI, PIP and PIP2 to talin and the effect of phosphoinositides and adenosinenucleotides on talin-induced actin polymerization. At physiological salt concentrations, talin coprecipitates with liposomes when containing phosphoinositides but not when containing PI. The nucleating effect of talin as reflected by a twofold increase of fluorescence during the polymerization of actin labelled with NBD is not inhibited by phosphoinositides. The polymerization of ADP-actin versus ATP-actin was investigated in the presence and absence of talin by NBD fluorescence. ADP-actin nucleation induced by talin is comparably efficient as with ATP-actin. These experimental findings in summary have implications when evaluating the role of talin during cell activation.

References

Apr 1, 1991·Journal of Muscle Research and Cell Motility·G Isenberg
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Jun 24, 1991·FEBS Letters·S KaufmannG Isenberg
Jul 31, 1991·Biochemical and Biophysical Research Communications·W H Goldmann, G Isenberg
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Sep 28, 1990·Biochemical and Biophysical Research Communications·M MugurumaT Fukazawa
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Citations

Oct 9, 2001·Trends in Biochemical Sciences·V Niggli
Jan 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·David T MooreWilliam F DeGrado
Apr 16, 2002·Molecular Biology of the Cell·Hélène DefacqueGareth Griffiths
Dec 8, 2005·Biochemistry·Roman BruneckyTatiana G Kutateladze
Feb 26, 2016·Cell Biology International·Wolfgang H Goldmann
Aug 29, 2006·Journal of Molecular Biology·Shuichi TakedaKingo Takiguchi
Sep 5, 2001·The Journal of Biological Chemistry·C C CunninghamP A Janmey
Mar 30, 2001·The Journal of Biological Chemistry·V MartelC Albiges-Rizo
Feb 25, 2005·The Journal of Biological Chemistry·Daniel M CohenSusan W Craig
May 21, 1999·Archives of Biochemistry and Biophysics·J M SchmidtR M Robson
Dec 5, 1998·Biochimica Et Biophysica Acta·K A HinchliffeR F Irvine

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