PROBING PLANT METABOLISM WITH NMR

Annual Review of Plant Physiology and Plant Molecular Biology
R G Ratcliffe, Yair Shachar-Hill

Abstract

Analytical methods for probing plant metabolism are taking on new significance in the era of functional genomics and metabolic engineering. Among the available methods, nuclear magnetic resonance (NMR) spectroscopy is a technique that can provide insights into the integration and regulation of plant metabolism through a combination of in vivo and in vitro measurements. Thus NMR can be used to identify, quantify, and localize metabolites, to define the intracellular environment, and to explore pathways and their operation. We review these applications and their significance from a metabolic perspective. Topics of current interest include applications of NMR to metabolic flux analysis, metabolite profiling, and metabolite imaging. These and other areas are discussed in relation to NMR investigations of intermediary carbon and nitrogen metabolism. We conclude that metabolic NMR has a continuing role to play in the development of a quantitative understanding of plant metabolism and in the characterization of metabolic phenotypes.

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Citations

Sep 7, 2005·Photosynthesis Research·F Mesnard, R G Ratcliffe
May 11, 2013·Photosynthesis Research·Carmela Rosaria GuadagnoNicola D'Ambrosio
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Methods Mentioned

BETA
nuclear magnetic resonance
NMR
transgenic
deamination
phosphotransferase
chemical shift

Software Mentioned

EXSY

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