Probing Polymer Chain Conformation and Fibril Formation of Peptide Conjugates

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Zhanna EvgrafovaJochen Balbach

Abstract

Covalent conjugates between a synthetic polymer and a peptide hormone were used to probe the molecular extension of these macromolecules and how the polymer modifies the fibril formation of the hormone. NMR spectroscopy of 15 N labeled parathyroid hormone (PTH) was employed to visualize the conformation of the conjugated synthetic polymer, triggered by small temperature changes via its lower critical solution temperature. A shroud-like polymer conformation dominated the molecular architecture of the conjugated chimeras. PTH readily forms amyloid fibrils, which is probably the physiological storage form of the hormone. The polyacrylate based polymers stimulated the nucleation processes of the peptide.

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Citations

Oct 22, 2019·Macromolecular Rapid Communications·Zhanna EvgrafovaWolfgang H Binder
Sep 19, 2019·Physical Chemistry Chemical Physics : PCCP·Zhanna EvgrafovaWolfgang H Binder
Jan 20, 2019·International Journal of Molecular Sciences·Amit KumarJochen Balbach
Sep 17, 2020·ACS Applied Materials & Interfaces·Valerie Ortiz-GómezEduardo Nicolau

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