Nov 26, 2018

Probing RNA Structure in Liquid-Liquid Phase Separation Using SHAPE-MaP

Methods in Enzymology
Erin M Langdon, Amy S Gladfelter

Abstract

RNA is an integral component of many biological condensates. A variety of features of RNAs are linked to their function in biological phase separation. Length and negative charge provide fairly generic chemical inputs that drive condensation while sequence has been shown to influence both the molecular identity and biophysical properties of droplets. mRNA sequence guides the secondary structure of the polymers and RNA secondary structure licenses-specific RNA-RNA interactions and the recruitment of RNA-binding proteins. Here, we describe a method for directly probing the structure of mRNAs in the context of RNP-droplets formed via LLPS.

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Mentioned in this Paper

Exploration With a Probe
Lipid Droplet
Microtubule-Associated Proteins
Alkalescens-Dispar Group
Transcription, Genetic
Proteins, Recombinant DNA
Immunofluorescence Microscopy
Gene Expression
Biophysics
Polymers

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