PMID: 7543200Jul 11, 1995Paper

Probing sequence-specific RNA recognition by the bacteriophage MS2 coat protein

Nucleic Acids Research
P G StockleyK Valegård

Abstract

We present the results of in vitro binding studies aimed at defining the key recognition elements on the MS2 RNA translational operator (TR) essential for complex formation with coat protein. We have used chemically synthesized operators carrying modified functional groups at defined nucleotide positions, which are essential for recognition by the phage coat protein. These experiments have been complemented with modification-binding interference assays. The results confirm that the complexes which form between TR and RNA-free phage capsids, the X-ray structure of which has recently been reported at 3.0 A, are identical to those which form in solution between TR and a single coat protein dimer. There are also effects on operator affinity which cannot be explained simply by the alteration of direct RNA-protein contacts and may reflect changes in the conformational equilibrium of the unliganded operator. The results also provide support for the approach of using modified oligoribonucleotides to investigate the details of RNA-ligand interactions.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1977·Nucleic Acids Research·H Donis-KellerW Gilbert
Mar 1, 1978·FEBS Letters·F Sanger, A R Coulson
Apr 11, 1992·Nucleic Acids Research·D S Peabody, K R Ely
Nov 25, 1987·Nucleic Acids Research·C EhresmannB Ehresmann
Dec 1, 1989·Journal of Molecular Graphics·I HaneefP G Stockley
Dec 20, 1988·Journal of Molecular Biology·D Beckett, O C Uhlenbeck
Mar 24, 1987·Biochemistry·P J RomaniukO C Uhlenbeck
May 14, 1967·Journal of Molecular Biology·T SugiyamaK A Hartman
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·O C UhlenbeckD Beckett
Apr 1, 1994·Analytical Biochemistry·J B MurrayJ R Arnold
Apr 1, 1993·The Journal of General Virology·R A MasticoP G Stockley
Aug 1, 1993·Biochemical Society Transactions·P G StockleyH K Tewary

❮ Previous
Next ❯

Citations

Dec 1, 1996·Protein Science : a Publication of the Protein Society·C Z NiK R Ely
Sep 16, 2010·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Thomas DangeDavid Grünwald
Jul 31, 2013·Quarterly Reviews of Biophysics·Michael G Rossmann
Nov 10, 1998·Nature Structural Biology·S RowsellS E Phillips
May 9, 2006·Nature Biotechnology·Farren J IsaacsJames J Collins
Feb 14, 1998·Nature Structural Biology·M A ConveryP G Stockley
Mar 21, 1998·Nature Structural Biology·O C Uhlenbeck
Feb 22, 2003·Nucleic Acids Research·Shamol SahaKevin A Jarell
Mar 2, 2013·Journal of Virology·Sujata HalderMavis Agbandje-McKenna
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·H E JohanssonO C Uhlenbeck
Jul 6, 2014·Plant Methods·Janett GöhringJaroslaw Jacak
Nov 27, 2015·Journal of Molecular Biology·Óttar RolfssonPeter G Stockley
Jul 21, 2012·Methods : a Companion to Methods in Enzymology·Je-Hyun YoonMyriam Gorospe
Dec 29, 2005·Journal of Molecular Biology·Dagmar Hobson, Olke C Uhlenbeck
Sep 6, 2005·Molecular and Biochemical Parasitology·Huafang ShiElisabetta Ullu
Feb 21, 2013·RNA Biology·Alexander BorodavkaPeter G Stockley
Jul 28, 2016·The Journal of Chemical Physics·Matthew R PerkettMichael F Hagan
Dec 6, 2001·Journal of Molecular Biology·D DertingerO C Uhlenbeck
Oct 25, 2011·Journal of the American Chemical Society·Zhixue ZhuHoward M Colquhoun
Nov 1, 2012·Molecular Pharmaceutics·Francis A Galaway, Peter G Stockley
Sep 30, 2010·Physical Chemistry Chemical Physics : PCCP·Victoria L MortonAlison E Ashcroft
Apr 6, 2019·Annual Review of Biophysics·Reidun Twarock, Peter G Stockley
Jan 23, 2018·Frontiers in Microbiology·Chun Shen Lim, Chris M Brown

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.