Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions

Nucleus
Tao DumurCélia Baroux

Abstract

The eukaryotic cell nucleus is a central organelle whose architecture determines genome function at multiple levels. Deciphering nuclear organizing principles influencing cellular responses and identity is a timely challenge. Despite many similarities between plant and animal nuclei, plant nuclei present intriguing specificities. Complementary to molecular and biochemical approaches, 3D microscopy is indispensable for resolving nuclear architecture. However, novel solutions are required for capturing cell-specific, sub-nuclear and dynamic processes. We provide a pointer for utilising high-to-super-resolution microscopy and image processing to probe plant nuclear architecture in 3D at the best possible spatial and temporal resolution and at quantitative and cell-specific levels. High-end imaging and image-processing solutions allow the community now to transcend conventional practices and benefit from continuously improving approaches. These promise to deliver a comprehensive, 3D view of plant nuclear architecture and to capture spatial dynamics of the nuclear compartment in relation to cellular states and responses. Abbreviations: 3D and 4D: Three and Four dimensional; AI: Artificial Intelligence; ant: antipodal nuclei (ant); C...Continue Reading

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Citations

Jun 21, 2020·International Journal of Molecular Sciences·Dominika Idziak-HelmckeEdyta Skrzypek
May 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lloyd Donaldson
Aug 18, 2020·Journal of Experimental Botany·Alžběta NěmečkováEva Hřibová
Aug 19, 2021·Trends in Plant Science·Agnese PiovesanBart Nicolaï
Oct 1, 2021·Journal of Experimental Botany·Katja Graumann

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Methods Mentioned

BETA
ChIP
transgenic
imaging technique
light-scattering
light scattering
optical microscopy
light diffraction
super resolution microscopy
fluorescence microscopy
confocal microscopy

Software Mentioned

Imaris
PALM
Autoquant
ImageJ
Hyvolution
PEA CLARITY
XTFISHInsideNucleus
Cell Profiler
STORM
TOMEI

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