Probing the adjustments of macromolecules during their surface adsorption

ACS Applied Materials & Interfaces
Pengxiang JiaJiang Zhao

Abstract

Thiol-terminated polymers poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC-SH), poly(N,N-isopropylacrylamide) (PNIPAM-SH), and poly(tert-butyl acrylate) (PtBA-SH) were synthesized, and the polymers were grafted on the gold surfaces of quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR) sensor chips to form brushes. The grafting process of the polymer brushes as well as protein adsorption onto the brush layers was monitored by in situ QCM-D and SPR techniques. By examining the changes in frequency and dissipation factor as well as the value of ∂D/∂f from QCM-D measurements, different stages of the polymer grafting and protein adsorption are distinguished. The most interesting discovery is the conformation change of BSA protein adsorption from a weakly adsorbed native state to a strongly immobilized denatured state on the polymer brushes. The corresponding change in BSA adsorption from a reversible state to an irreversible state was confirmed by SPR measurements. The adsorption of protein on the polymer brushes' surface relies largely on interaction between the protein and the polymers, and the stronger hydrophilicity of the surfaces is proved to be more effective to suppress the protein a...Continue Reading

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Citations

Sep 30, 2016·Journal of Colloid and Interface Science·Alejandro BarrantesHåvard J Haugen
Nov 15, 2018·Angewandte Chemie·Yinqiang XiaXavier Banquy
Mar 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Silke HeinenMarie Weinhart
Feb 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wei DaiJiang Zhao
Jan 20, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fang LiuHanying Zhao
Dec 19, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaowen SunChun Feng
Aug 25, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Athénaïs DavantèsDenis Renard
Aug 28, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Hai-Xia WuChuan-Jun Liu

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