Probing the circadian control of phosphoenolpyruvate carboxylase kinase expression in Kalanchoë fedtschenkoi

Functional Plant Biology : FPB
James HartwellHugh G Nimmo

Abstract

This paper originates from a presentation at the IIIrd International Congress on Crassulacean Acid Metabolism, Cape Tribulation, Queensland, Australia, August 2001. In crassulacean acid metabolism (CAM) plants, phosphoenolpyruvate carboxylase (PEPC) kinase is expressed at night under the control of a circadian oscillator. We have proposed that this is an indirect effect, secondary to circadian fluctuations in the level of a metabolite, possibly cytosolic malate, resulting from a primary effect on the permeability of the tonoplast (Nimmo 2000, Trends in Plant Science 5, 75-80). Here we show that the nocturnal accumulation of PEPC kinase translatable mRNA and phosphorylation of PEPC in Kalanchoë fedtschenkoi is blocked by the protein phosphatase inhibitor cantharidin. This implicates protein dephosphorylation in the circadian pathway that regulates expression of PEPC kinase. We also show that the effect of reducing the temperature from 30 to 15 °C on CO2 fixation by detached leaves held in constant darkness and normal air is 'gated' by the circadian clock. This strongly supports the view that the effect of the clock on the expression of PEPC kinase is secondary rather than direct. We have developed a non-aqueous fractionation pro...Continue Reading

Citations

Jun 10, 2006·The Plant Journal : for Cell and Molecular Biology·Hiroshi FukayamaHugh G Nimmo
Apr 28, 2009·Journal of Experimental Botany·Anne M BorlandJ Andrew C Smith
Apr 26, 2008·Journal of Experimental Botany·Ulrich Lüttge
Aug 11, 2006·The Plant Journal : for Cell and Molecular Biology·Michael J WilkinsonC Nicholas Hewitt
Apr 16, 2016·Current Opinion in Plant Biology·James HartwellSusanna F Boxall
Nov 1, 2003·The New Phytologist·Alex A R Webb

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