Probing the Conformation-Dependent Preferential Binding of Ethanol to Cationic Glycylalanylglycine in Water/Ethanol by Vibrational and NMR Spectroscopy

The Journal of Physical Chemistry. B
David DiGuiseppiReinhard Schweitzer-Stenner

Abstract

The conformational propensity of amino acid residues is determined by an intricate balance of peptide-solvent and solvent-solvent interactions. To explore how the systematic replacement of water by a cosolvent affects the solvation of both the amino acid backbone and side chains, we performed a combined vibrational spectroscopy and NMR study of cationic glycylalanylglycine (GAG) in different ethanol/water mixtures of between 0 and 42 mol percent ethanol. Classical model peptide N'-methylacetamide was used as a reference system to probe solvent-induced spectroscopic changes. The alanine residue of GAG in water is known to exhibit a very high propensity for polyproline II (pPII). Adding up to 30 mol % ethanol at room temperature leads only to minor changes in the Ramachandran distribution of alanine, which mostly changes within the individual conformational subspaces. A further increase in the ethanol fractions leads to a destabilization of pPII and a stabilization of β-strand conformations. At higher temperatures, different degrees of enthalpy-entropy compensations lead to a much stronger influence of ethanol on the peptide's conformational distribution. Ethanol-induced changes in chemical shifts and amide I wavenumbers strongly...Continue Reading

References

Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·S N Timasheff
Jun 1, 1997·Journal of Biomolecular NMR·N J Baxter, M P Williamson
Feb 12, 1998·Journal of Molecular Biology·C J PenkettL J Smith
Jun 14, 2002·Journal of Biomolecular NMR·Julia Wirmer, Harald Schwalbe
Jul 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhengshuang ShiNeville R Kallenbach
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Serge N Timasheff
Nov 28, 2002·Journal of the American Chemical Society·Fatma EkerReinhard Schweitzer-Stenner
May 23, 2003·Protein Science : a Publication of the Protein Society·Matthew P Hinderaker, Ronald T Raines
Jul 1, 1963·Journal of Molecular Biology·G N RAMACHANDRANV SASISEKHARAN
Oct 24, 2003·Protein Science : a Publication of the Protein Society·Bosco K HoRobert Brasseur
Feb 26, 2004·Journal of the American Chemical Society·Alexander N DrozdovRohit V Pappu
Mar 5, 2004·Journal of the American Chemical Society·Reinhard Schweitzer-StennerLaurence A Nafie
Apr 22, 2004·Journal of the American Chemical Society·Iain H McCollLaurence D Barron
Jul 23, 2004·The Journal of Chemical Physics·Jun-Ho Choi, Minhaeng Cho
Nov 19, 2004·Journal of the American Chemical Society·Zhigang LiuNeville R Kallenbach
Jan 27, 2005·Journal of Molecular Biology·Martino CalamaiChristopher M Dobson
Sep 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Abhishek K JhaTobin R Sosnick
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zhengshuang ShiNeville R Kallenbach
Apr 18, 2006·PLoS Computational Biology·Bosco K Ho, Ken A Dill
May 11, 2006·Chemical Reviews·Zhengshuang ShiNeville R Kallenbach
Feb 1, 2007·Journal of the American Chemical Society·Jürgen GrafHarald Schwalbe
Feb 17, 2007·The Journal of Chemical Physics·Roman D GorbunovGerhard Stock
Apr 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Reinhard Schweitzer-Stenner, Thomas J Measey
Feb 12, 2008·The Journal of Physical Chemistry. B·Joze GrdadolnikFranc Avbelj
Jul 18, 2008·Science Signaling·R Adron HarrisS John Mihic
Dec 18, 2009·Journal of the American Chemical Society·Andrew HagarmanReinhard Schweitzer-Stenner
Apr 29, 2010·The Journal of Physical Chemistry. B·R C Neuman, J T Gerig

❮ Previous
Next ❯

Citations

Apr 27, 2018·Physical Chemistry Chemical Physics : PCCP·Niranjan V IlaweBryan M Wong
Aug 6, 2020·Biomolecules·Brian AndrewsBrigita Urbanc
Feb 24, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Lavenia J ThurschNicolas J Alvarez
Apr 12, 2020·Journal of Colloid and Interface Science·David M DiGuiseppiReinhard Schweitzer-Stenner
Jun 17, 2021·The Journal of Physical Chemistry. B·Reinhard Schweitzer-Stenner, Nicolas J Alvarez
Dec 11, 2020·The Journal of Physical Chemistry. B·Shuting ZhangBrigita Urbanc
Jul 25, 2019·The Journal of Physical Chemistry. B·Arusha AcharyyaTimothy D Vaden
Dec 29, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Daria MaltsevaGrazia Gonella

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.