Probing the (H3-H4)2 histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling.

Nucleic Acids Research
Andrew BowmanDavid George Norman

Abstract

The (H3-H4)(2) histone tetramer forms the central core of nucleosomes and, as such, plays a prominent role in assembly, disassembly and positioning of nucleosomes. Despite its fundamental role in chromatin, the tetramer has received little structural investigation. Here, through the use of pulsed electron-electron double resonance spectroscopy coupled with site-directed spin labelling, we survey the structure of the tetramer in solution. We find that tetramer is structurally more heterogeneous on its own than when sequestered in the octamer or nucleosome. In particular, while the central region including the H3-H3' interface retains a structure similar to that observed in nucleosomes, other regions such as the H3 alphaN helix display increased structural heterogeneity. Flexibility of the H3 alphaN helix in the free tetramer also illustrates the potential for post-translational modifications to alter the structure of this region and mediate interactions with histone chaperones. The approach described here promises to prove a powerful system for investigating the structure of additional assemblies of histones with other important factors in chromatin assembly/fluidity.

References

Jul 1, 1977·Cell·M Bina-Stein, R T Simpson
May 28, 1982·Science·E LattmanE N Moudrianakis
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A FlausT J Richmond
Nov 5, 1997·Journal of Molecular Biology·K LugerT J Richmond
Feb 20, 1999·Cell·Y LorchR D Kornberg
Feb 13, 2002·Current Opinion in Structural Biology·Warren L DeLano
Apr 12, 2002·Journal of Magnetic Resonance·Gunnar JeschkeAdelheid Godt
Dec 31, 2002·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Gunnar Jeschke
Feb 5, 2003·Journal of Magnetic Resonance·Charles D SchwietersG Marius Clore
Dec 24, 2003·Molecular Cell·Michael BrunoTom Owen-Hughes
Apr 21, 2004·Protein Science : a Publication of the Protein Society·Douglas D Banks, Lisa M Gloss
May 6, 2004·Journal of the American Chemical Society·Olav SchiemannThomas F Prisner
Jul 24, 2004·Acta Crystallographica. Section D, Biological Crystallography·Alexander W Schüttelkopf, Daan M F van Aalten
Jul 30, 2004·Nature·Ben E BlackDon W Cleveland
Dec 8, 2004·Nature Structural & Molecular Biology·Gu LiJonathan Widom
Jan 15, 2005·Journal of Magnetic Resonance·Yun-Wei ChiangJack H Freed
Mar 17, 2005·Genes & Development·Christophe Thiriet, Jeffrey J Hayes
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Christopher M WoodJohn P Baldwin
Jul 25, 2006·Nature·Eran SegalJonathan Widom
Nov 4, 2006·Cell·Christine M EnglishJessica K Tyler
Feb 27, 2007·Molecular Cell·Toshiaki TsubotaPaul D Kaufman
Mar 3, 2007·The EMBO Journal·Susan M HansonVsevolod V Gurevich
Mar 10, 2007·Science·Michael F DionOliver J Rando
Mar 21, 2007·The Journal of Biological Chemistry·Junhong HanZhiguo Zhang
Mar 28, 2007·Molecular and Cellular Biology·Helder FerreiraTom Owen-Hughes
Jun 15, 2007·Quarterly Reviews of Biophysics·Olav Schiemann, Thomas F Prisner
Jul 7, 2007·Molecular Cell·Aurélien BancaudAriel Prunell
Aug 7, 2007·PLoS Biology·Yamini DalalSteven Henikoff
Sep 22, 2007·Chembiochem : a European Journal of Chemical Biology·Richard WardDavid G Norman

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Citations

Jun 5, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Gunnar Jeschke
Jan 11, 2012·Journal of the American Chemical Society·Xiaojun ZhangPeter Z Qin
Nov 28, 2012·Nature Structural & Molecular Biology·Wei ZhangErnest D Laue
Nov 19, 2013·Analytical Biochemistry·Qinqin ZhuangChristopher M Wood
May 3, 2014·Chromosoma·Antonia P M Jack, Sandra B Hake
Aug 6, 2014·Journal of Biotechnology·Xiangyong LiuZhaojie Zhang
Mar 27, 2012·Applied Magnetic Resonance·Gregor HageluekenOlav Schiemann
Aug 27, 2013·Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration·Caroline IngrePeter M Andersen
Oct 21, 2014·Chemical Reviews·Manuel M Müller, Tom W Muir
Aug 4, 2011·The FEBS Journal·Andrew Flaus, Tom Owen-Hughes
Feb 26, 2011·The Biochemical Journal·Gunnar W Reginsson, Olav Schiemann
Sep 2, 2010·Journal of Magnetic Resonance·Richard WardDavid G Norman
Oct 16, 2014·Nature Reviews. Molecular Cell Biology·Peter Tessarz, Tony Kouzarides
Jun 13, 2015·Royal Society Open Science·Hugh P MorganMalcolm D Walkinshaw
Aug 26, 2016·Scientific Reports·Noelia Fernández-RiveroAdelina Prado
Jan 6, 2017·Nature Reviews. Molecular Cell Biology·Colin M HammondAnja Groth
Sep 13, 2014·BioMed Research International·Maxim V ZagoskinDmitry V Mukha
Sep 1, 2013·Insects·Ángel M Casanova-Torres, Heidi Goodrich-Blair
May 11, 2017·Cold Spring Harbor Molecular Case Studies·Andrew V UzilovEliza B Geer

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Methods Mentioned

BETA
electron-electron double
gel filtration
size exclusion chromatography
circular dichroism
acetylation

Software Mentioned

DeerAnalysis2006
PyMol
Ghemical
MTSSL
PELDOR
SDSL
PRODG
DeerAnalysis

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