Probing the hydrophobic effect of noncovalent complexes by mass spectrometry.

Journal of the American Society for Mass Spectrometry
Claudia BichR Zenobi

Abstract

The study of noncovalent interactions by mass spectrometry has become an active field of research in recent years. The role of the different noncovalent intermolecular forces is not yet fully understood since they tend to be modulated upon transfer into the gas phase. The hydrophobic effect, which plays a major role in protein folding, adhesion of lipid bilayers, etc., is absent in the gas phase. Here, noncovalent complexes with different types of interaction forces were investigated by mass spectrometry and compared with the complex present in solution. Creatine kinase (CK), glutathione S-transferase (GST), ribonuclease S (RNase S), and leucine zipper (LZ), which have dissociation constants in the nM range, were studied by native nanoelectrospray mass spectrometry (nanoESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with chemical cross-linking (XL). Complexes interacting with hydrogen bonds survived the transfer into gas phase intact and were observed by nanoESI-MS. Complexes that are bound largely by the hydrophobic effect in solution were not detected or only at very low intensity. Complexes with mixed polar and hydrophobic interactions were detected by nanoESI-MS, most likely du...Continue Reading

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Citations

Dec 7, 2013·Analytical Chemistry·Lars KonermannModupeola A Sowole
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Jiangjiang Liu, Lars Konermann
Jan 25, 2012·Journal of the American Society for Mass Spectrometry·Elena N KitovaJohn S Klassen
Aug 17, 2010·Journal of the American Society for Mass Spectrometry·Stefanie MädlerRenato Zenobi
Dec 14, 2011·Journal of Medicinal Chemistry·Hannah J MapleJohn Crosby
Dec 27, 2011·Organic Letters·José P Da SilvaV Ramamurthy
Feb 10, 2012·Journal of the Royal Society, Interface·Gillian R Hilton, Justin L P Benesch
Jun 30, 2014·Colloids and Surfaces. B, Biointerfaces·Dolores C CarrerEder Lilia Romero
Mar 1, 2012·Rapid Communications in Mass Spectrometry : RCM·Yingzhi LiuXian Wang
Feb 14, 2015·Protein Science : a Publication of the Protein Society·Elisabetta Boeri Erba, Carlo Petosa
May 4, 2016·Chemical Reviews·Frank Biedermann, Hans-Jörg Schneider
Aug 3, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Liliana Pedro, Ronald J Quinn
Nov 3, 2016·Journal of the American Chemical Society·Wei-Guang Seetoh, Chris Abell
Oct 21, 2015·Organic & Biomolecular Chemistry·Enrico FaggiIgnacio Alfonso
Apr 23, 2013·The Journal of Endocrinology·A P Santos-SilvaP C Lisboa
May 28, 2014·Rapid Communications in Mass Spectrometry : RCM·Hannah J MapleRebecca J Burnley
May 4, 2020·Journal of Proteomics·Elisabetta Boeri ErbaCarlo Petosa
May 27, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Jason C CrackNick E Le Brun
Oct 8, 2015·Journal of the American Society for Mass Spectrometry·Ekaterina KovalenkoVladimir P Fedin
Jun 1, 2017·Journal of the American Society for Mass Spectrometry·Marina RautenbachMarietjie A Stander
Jul 16, 2017·Journal of the American Society for Mass Spectrometry·Lars Konermann
Feb 7, 2019·The Journal of Physical Chemistry. B·Maryam Bakhtiari, Lars Konermann
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