Probing the influence of myelin and glia on the tensile properties of the spinal cord

Biomechanics and Modeling in Mechanobiology
David I ShreiberRagi A I Elias

Abstract

Although glia have been historically classified as the structurally supporting cells of the central nervous system, their role in tissue mechanics is still largely unstudied. The influence of myelin and glia on the mechanical properties of spinal cord tissue was examined by testing embryonic day 18 chick embryo spinal cords in uniaxial tension following disruption of the glial matrix using either ethidium bromide (EB) or an antibody against galactocerebroside (alphaGalC) in the presence of complement. Demyelination was confirmed by myelin basic protein immunoreactivity and quantified using osmium tetroxide staining. A substantial loss of astrocytes and oligodendrocytes concurrent with demyelination was observed following EB injection but not alphaGalC injection. No morphological changes were observed following injection of saline or IgG with complement as controls for EB and alphaGalC. Demyelinated spinal cords demonstrated significantly lower stiffness and ultimate tensile stress than myelinated spinal cords. No significant differences were observed in the tensile response between the two demyelinating protocols. The results demonstrate that the glial matrix provides significant mechanical support to the spinal cord, and sugge...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H S KeirsteadJ D Steeves
Jan 1, 1989·Acta Neuropathologica·K OzawaM Kameyama
May 19, 1987·Brain Research·W M CarrollF L Mastaglia
Nov 1, 1986·Neuropathology and Applied Neurobiology·D L Graça, W F Blakemore
Jan 1, 1986·Journal of Biomechanics·D I BylskiJ W Melvin
Jun 1, 1984·Journal of the Neurological Sciences·R C SergottR P Lisak
Jan 1, 1996·Annals of Biomedical Engineering·L E Bilston, L E Thibault
Dec 1, 1996·Methods : a Companion to Methods in Enzymology·R ReynoldsS Piddlesden
Jan 1, 1997·Brain Research Bulletin·H S KeirsteadJ D Steeves
Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E M Quigley, J S Thompson
Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C G FernandesL A Pereira
Aug 5, 1999·Journal of Biomechanics·K B Arbogast, S S Margulies
May 16, 2001·Biomaterials·A P BalgudeR V Bellamkonda
Dec 25, 2002·Neuroreport·Lisa A FlanaganPaul A Janmey
Oct 16, 2003·The Journal of Surgical Research·Gregory H BorschelRobert G Dennis
Feb 28, 2004·Journal of Biomechanical Engineering·Allison C BainDavid F Meaney
Jul 19, 2005·Nature Reviews. Neuroscience·Andrea Volterra, Jacopo Meldolesi
Aug 3, 2005·Journal of Biomechanical Engineering·A GefenS S Margulies
Sep 2, 2005·Nature Reviews. Neuroscience·Diane L Sherman, Peter J Brophy
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Nov 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yun-Bi LuAndreas Reichenbach
May 24, 2007·Journal of Neurotrauma·Benjamin S ElkinBarclay Morrison
Jul 28, 2007·Journal of Biomechanical Engineering·Hailing Hao, David I Shreiber

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Citations

Jun 9, 2010·Journal of Biomechanical Engineering·Benjamin S ElkinBarclay Morrison
May 7, 2013·Annual Review of Biomedical Engineering·Kristian FranzeJochen Guck
Dec 18, 2013·Biomechanics and Modeling in Mechanobiology·Antoine JérusalemJosé M Peña
Jul 7, 2010·Acta Biomaterialia·Thibault P PrevostSimona Socrate
Aug 2, 2011·IEEE Transactions on Bio-medical Engineering·Yi PanAssimina A Pelegri
Sep 30, 2015·The Journal of Cell Biology·Ryan B MacDonaldWilliam A Harris
Apr 26, 2015·Biomechanics and Modeling in Mechanobiology·Sagar SinghDavid I Shreiber
May 9, 2015·Biophysical Journal·David E KoserKristian Franze
May 21, 2014·Journal of Biomechanics·Xiaofei WangChristian J Lueck
May 24, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Kevin M Labus, Christian M Puttlitz
Sep 7, 2016·Regenerative Medicine·Richard D BartlettJames B Phillips
May 2, 2020·The European Journal of Neuroscience·Chloe M HallGraham K Sheridan
Sep 27, 2019·Journal of Biomechanical Engineering·Annaclaudia MontaninoSvein Kleiven
Jun 5, 2020·Annals of Biomedical Engineering·Faezeh EskandariKaveh Laksari
Dec 20, 2019·Biomechanics and Modeling in Mechanobiology·Seyed Abdolmajid YousefsaniFarzam Farahmand
Dec 2, 2020·Neurobiology of Disease·Carolyn E Keating, D Kacy Cullen
Jul 1, 2020·Physics in Medicine and Biology·Daniel J SullivanAssimina A Pelegri
Dec 16, 2019·Neuroscience Letters·Ekta P MakhijaKrystyn J Van Vliet
Dec 31, 2019·Acta Biomaterialia·S BuddayG A Holzapfel

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