Probing the kinetic cooperativity of beta-sheet folding perpendicular to the strand direction

Biochemistry
Yao XuFeng Gai

Abstract

In an attempt to determine how the folding dynamics of multistranded beta-sheets vary with the strand number, we have studied the temperature-induced relaxation kinetics of a four-stranded beta-sheet, DPDPDP. Our results show that the thermally induced relaxation of DPDPDP occurs on the nanosecond time scale; however, a comparison of the current results with those obtained on a sequence-related, three-stranded beta-sheet suggests that increasing the strand number from three to four increases the folding free energy barrier by a minimum of 0.8 kcal/mol, depending on the folding mechanism. Therefore, these results together suggest that the relaxation kinetics of DPDPDP can be analyzed according to a two-state model even though its folding may actually involve parallel (but degenerate or nearly degenerate) kinetic pathways. The apparent, two-state folding time of DPDPDP is determined to be approximately 0.44 micros at the thermal melting temperature, which makes it one of the fastest folders known to date.

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Citations

May 17, 2011·The Journal of Physical Chemistry. B·Arnaldo L SerranoFeng Gai
Apr 2, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jan Kubelka
Apr 29, 2009·International Journal of Molecular Sciences·Vincent A VoelzVijay S Pande
Feb 4, 2016·Chemical Reviews·A Subha Mahadevi, G Narahari Sastry
Aug 22, 2015·Biomacromolecules·Clemens N Z SchmittMatthew J Harrington
Dec 17, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Elena ColangeloRaphaël Lévy
Aug 18, 2009·The Journal of Physical Chemistry. B·Daniel L Ensign, Vijay S Pande
Feb 16, 2011·The Journal of Physical Chemistry. B·Travis Hoppe, Jian-Min Yuan
Mar 26, 2013·Biochemistry·Michele ScianNiels H Andersen
Mar 13, 2014·The Journal of Physical Chemistry. B·Beatrice N MarkiewiczFeng Gai

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