Probing the mechanism of aqueous two-phase system formation for alpha-elastin on-chip

Journal of the American Chemical Society
Yanjie ZhangPaul S Cremer

Abstract

The kinetics of formation of the aqueous two-phase system (ATPS) for alpha-elastin was studied by dark field microscopy in an on-chip linear temperature gradient. Scattering intensities of protein solutions were recorded as a function of temperature and time, simultaneously at several concentrations. It was found that the formation rate of the ATPS could be fit as a first-order process and that the apparent rate constant increased with protein concentration. The activation energy for the process was 9.5 +/- 0.5 kcal/mol, and this result was consistent with a coalescence mechanism. Experiments were also conducted with varying concentrations of sodium dodecyl sulfate, which shut off the coalescence mechanism forcing ATPS formation to proceed through Ostwald ripening. When this was done, the activation energy increased to 33 +/- 2 kcal/mol and the kinetics became consistent with a second-order process.

References

Jul 19, 1976·Biochimica Et Biophysica Acta·D VolpinL Gotte
Apr 1, 1971·The Journal of Pharmacy and Pharmacology·A T Florence, J A Rogers
Feb 7, 1998·European Journal of Biochemistry·B VrhovskiA S Weiss
Aug 12, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J C MerchukJ A Asenjo
Aug 12, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M H SalamancaJ A Asenjo
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·C Solano-Castillo, M Rito-Palomares
Nov 25, 2000·Applied Microbiology and Biotechnology·J SinhaT Panda
Nov 28, 2001·Molecular Biotechnology·R Hatti-Kaul

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Citations

Jun 6, 2009·Journal of the American Chemical Society·Xin GeCarlos D M Filipe
Nov 19, 2009·Journal of the American Chemical Society·Younhee ChoPaul S Cremer
Oct 10, 2008·The Journal of Physical Chemistry. B·Younhee ChoPaul S Cremer
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yanjie Zhang, Paul S Cremer
Mar 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·M Scott LongChristine D Keating
Mar 31, 2005·Annual Review of Physical Chemistry·Matthew A Holden, Paul S Cremer
Jan 6, 2017·Journal of the American Chemical Society·Jan HeydaPaul S Cremer
Jul 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Bradley A RogersPaul S Cremer

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