Probing the phagolysosomal environment of human macrophages with a Ca2+-responsive operon fusion in Yersinia pestis

Nature
C PollackM S Klempner

Abstract

Several microorganisms, including Yersinia sp., Salmonella sp., Brucella sp., Mycobacterium sp. and Leishmania sp., have successfully adapted to grow within macrophage phagolysosomes. Infections caused by these intracellular pathogens are among the most difficult to treat. As part of an antimicrobial strategy directed at modifying the phagolysosomal environment to the disadvantage of these important pathogens, we are defining the ambient conditions within the organism-containing phagolysosome. To probe this environment, we have used Yersinia pestis, whose expression of several virulence attributes is highly dependent on the Ca2+ concentration in its growth environment. We first genetically engineered a strain of Y. pestis which responds to a low-calcium environment by transcription of inserted structural genes of the Escherichia coli lac operon. Using this mutant organism as a relevant biological probe, we demonstrate here that the calcium concentration in Y. pestis-containing phagolysosomes is sufficiently low to permit virulence gene expression; this resolves the question of where Y. pestis might express its Ca2+-regulated genes in vivo.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M J Casadaban, S N Cohen
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J HorstW Gerok
May 5, 1985·Nature·L D SibleyJ L Krahenbuhl
Jul 1, 1985·The Journal of Clinical Investigation·M S Klemper
Jan 1, 1984·The Journal of Clinical Investigation·H LagastF A Waldvogel
Sep 1, 1984·Infection and Immunity·S C Straley, P A Harmon
Sep 1, 1983·The Journal of Infectious Diseases·M S KlempnerD J Wyler
Feb 1, 1959·The Biochemical Journal·J CONCHIEG A LEVVY

❮ Previous
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Citations

Feb 24, 1988·Biochimica Et Biophysica Acta·P Elsbach, J Weiss
Nov 1, 1993·Trends in Microbiology·S C Straley
Jun 12, 2002·Current Opinion in Microbiology·Johan H J Leveau, Steven E Lindow
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J B BliskaS Falkow
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M Alpuche ArandaS I Miller
Jan 1, 1987·Critical Reviews in Plant Sciences·B W Poovaiah, A S Reddy
Dec 17, 2003·Current Opinion in Hematology·Henry Rosen
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H Smith
May 1, 1995·Biological Reviews of the Cambridge Philosophical Society·H Smith
Oct 25, 1996·Annals of the New York Academy of Sciences·H Smith
Dec 12, 2012·Journal of Bacteriology·Katherine Given LigtenbergOlaf Schneewind
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Kumaran S Ramamurthi, Olaf Schneewind
Oct 1, 1991·The Journal of Clinical Investigation·R T Ellison, T J Giehl
Apr 1, 1993·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·G R Cornelis
Dec 1, 1991·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·F G AbathL C Ferreira
Nov 4, 2008·Molecular Microbiology·Bill BlaylockOlaf Schneewind
Oct 12, 2004·Biochemical and Biophysical Research Communications·Cameron E FordeSandra L McCutchen-Maloney
Mar 1, 1989·Microbial Pathogenesis·R J MehighR R Brubaker
Jun 1, 1988·Microbial Pathogenesis·M P Sory, G Cornelis
Jan 1, 1988·Society for Applied Bacteriology Symposium Series·P H WilliamsG Hinson
Apr 20, 2006·Experimental Parasitology·Reynolds K B Brobey, Lynn Soong
Aug 13, 2005·Cell·Martin W BaderSamuel I Miller
Feb 2, 2006·PLoS Pathogens·Nihal A OkanA Wali Karzai
Oct 13, 2000·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·S NomuraS Fukuhara
Oct 1, 1989·Molecular Microbiology·G R CornelisJ C Vanooteghem
Jun 1, 1989·Microbiological Reviews·B B Finlay, S Falkow
Jul 1, 1992·Journal of Bacteriology·N P HoeJ D Goguen
Jan 1, 1997·Clinical Microbiology Reviews·R D Perry, J D Fetherston
Jul 1, 1993·Infection and Immunity·P S HiemstraR I Lehrer
Jan 1, 1993·Infection and Immunity·R J Mehigh, R R Braubaker

❮ Previous
Next ❯

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