PMID: 8962674Nov 1, 1995Paper

Probing the presumed catalytic triad of selenium-containing peroxidases by mutational analysis of phospholipid hydroperoxide glutathione peroxidase (PHGPx)

Biological Chemistry Hoppe-Seyler
Matilde MaiorinoLeopold Flohé

Abstract

Single and double site mutants affecting the presumed catalytic centre of the selenoenzyme PHGPx were subjected to functional analysis. The rate constants k+1 and k'+2, for the oxidation and the regeneration of the ground state enzyme were estimated, respectively. Moreover, the alkylation rate of the reactive centre by iodoacetate (kinact.) was also analysed. The substitution of the catalytically competent selenocysteine 46 by cysteine (PHGPxcys46) decreased k+1 and k'+2 by about three orders of magnitude, although leaving unaffected kinact.. Furthermore, mutations of PHGPxcys46 involving the other residues of the triad decreased both kinact. and k+1, thus highlighting the involvement of Gln 81 and Trp 136 in the dissociation/activation of the nucleophilic cysteine thiol. In general, substitutions of Gln 81 or Trp 136 by acidic residues in PHGPxcys46 most dramatically depressed the k+1 values, because they practically prevented the dissociation of the thiol group, while neutral or positively charged residues in these positions allowed an intermediate dissociation and induced a corresponding reactivity of the thiol. Our data, for the first time, reveal that the presumed triad of selenocysteine, glutamine and tryptophan residues ...Continue Reading

References

May 1, 1992·European Journal of Biochemistry·C RocherJ Chaudière
Dec 1, 1991·Trends in Biochemical Sciences·A BöckC Baron
Jan 1, 1990·Methods in Enzymology·M MaiorinoF Ursini
Mar 29, 1985·Biochimica Et Biophysica Acta·F UrsiniC Gregolin
Jun 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·L FlohéE Eichele
Jun 1, 1983·European Journal of Biochemistry·O EppA Wendel
Jan 1, 1995·Methods in Enzymology·F UrsiniL Flohé
Jun 30, 1993·Biochemical and Biophysical Research Communications·R A SundeM Sugimoto
Apr 1, 1986·Journal of Computational Chemistry·Scott J WeinerDavid A Case

❮ Previous
Next ❯

Citations

Dec 28, 1999·BioFactors·K Wingler, R Brigelius-Flohé
Sep 25, 2001·BioFactors·A RoveriM Maiorino
Aug 5, 2003·BioFactors·Regina Brigelius-Flohé, Leopold Flohé
Jun 1, 2005·Biochimica Et Biophysica Acta·Stephane HerbettePatricia Roeckel-Drevet
Mar 31, 2004·Medical Hypotheses·Harold D Foster
May 29, 2003·Journal of Insect Physiology·D LiO Schmidt
Jul 11, 2002·Free Radical Biology & Medicine·Hartmut Kühn, Astrid Borchert
May 8, 1999·Free Radical Biology & Medicine·P SteinertL Flohé
Nov 24, 1999·Free Radical Biology & Medicine·L FlohéP Steinert
Nov 24, 1999·Free Radical Biology & Medicine·R Brigelius-Flohé
May 9, 2002·Journal of Biotechnology·Frieder W SchellerVadim Fridman
Aug 8, 2008·The Journal of Biological Chemistry·Johannes MelchersClaudia Muhle-Goll
Jul 24, 2010·Antioxidants & Redox Signaling·Leopold FlohéFulvio Ursini
Jun 3, 2009·Molecular Biology and Evolution·Sergi CastellanoAndrew G Clark
Feb 28, 2003·Biology of Reproduction·Matilde MaiorinoLeopold Flohe
Jan 29, 2014·Molekuliarnaia biologiia·E G VarlamovaE E Fecenko
Dec 12, 2012·Cancer Research·Caitlyn W BarrettChristopher S Williams
Jun 24, 2009·The Journal of Clinical Investigation·Eléonore ChaboryPatrick Vernet
May 20, 2003·Biological Chemistry·Pierluigi MauriFulvio Ursini
May 22, 2008·Genetics·Régine DayerStéphane D Lemaire
Nov 26, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Emmanouil E MalandrakisPanagiota Panagiotaki
Mar 19, 2013·Free Radical Biology & Medicine·Laura Orian, Stefano Toppo
Jun 15, 1996·European Journal of Biochemistry·M MaiorinoL Flohé
Jan 31, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Peter A Bain, Kathryn A Schuller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.