Probing the role of positive residues in the ADP/ATP carrier from yeast. The effect of six arginine mutations of oxidative phosphorylation and AAC expression

Biochemistry
V MüllerM Klingenberg

Abstract

ADP/ATP transport is the terminal step of oxidative phosphorylation in mitochondria. In this paper seven mutants of AAC2 from Saccharomyces cerevisiae are studied on the cellular and mitochondrial level. Six conspicuously located arginines were mutated into mostly neutral residues [Nelson, D. R., Lawson, J. E., Klingenberg, M., & Douglas, M. G. (1993) J. Mol. Biol. 230, 1159-1170]. R96A, R96H, R204L, and R294A are located in the second transmembrane helix of each repeat while R252I, R253I, and R254I are in the arginine triplet of the last domain. All six arginine residues are conserved in all known ADP/ATP carrier sequences. At the cellular level, oxidative phosphorylation in R96H and R294A retains 8% of the wild-type rate, but it is virtually zero in the other mutants. However, cytochrome c, a parameter of oxidative capacity, remains at 4-42% of wt. The weak coordination of respiratory chain and AAC expression indicates that respiration is needed also for other purposes. In mitochondria the AAC-linked ATP synthesis is measured and segregated by using the AAC inhibitor bongkrekate (BKA). Only the R96H and R294A mutants express a significant rate of AAC-dependent ATP synthesis amounting to 2-18% of the plasmid-borne wild-type AA...Continue Reading

References

Mar 17, 1975·European Journal of Biochemistry·M KlingenbergB Scherer
Jan 15, 1976·FEBS Letters·W BabelM Klingenberg
Apr 1, 1989·Archives of Biochemistry and Biophysics·M Klingenberg
Apr 5, 1983·European Journal of Biochemistry·M KlingenbergH Aquila
Oct 1, 1993·Journal of Bioenergetics and Biomembranes·M Klingenberg

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Citations

May 23, 2002·Biochimie·Anne-Sophie BelzacqCatherine Brenner
Feb 3, 2006·Annual Review of Biochemistry·H NuryE Pebay-Peyroula
Sep 22, 2001·Archives of Biochemistry and Biophysics·S G HuangM Klingenberg
Aug 31, 2001·Biochemical and Biophysical Research Communications·T HatanakaH Terada
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yi Wang, Emad Tajkhorshid
Mar 27, 2001·Biochemical and Biophysical Research Communications·E WinklerT Caskey
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·V DolceJ E Walker
Sep 11, 2014·Orphanet Journal of Rare Diseases·Matthias R BaumgartnerAnupam Chakrapani
Sep 10, 2008·The Journal of Cell Biology·Steven M ClaypoolCarla M Koehler
Feb 29, 2000·Biochimica Et Biophysica Acta·V TrézéguetG J Lauquin
May 31, 2008·Biochimica Et Biophysica Acta·Martin Klingenberg
Feb 16, 2019·Frontiers in Cell and Developmental Biology·Oluwaseun B Ogunbona, Steven M Claypool
Jun 6, 1998·Journal of Molecular Biology·D R NelsonJ M Swanson
Jan 16, 1999·Biochimica Et Biophysica Acta·M Klingenberg, S G Huang
Jun 9, 2006·Biochimica Et Biophysica Acta·Edmund R S Kunji, Alan J Robinson
Nov 9, 2005·Biochemistry·Vincent PostisVéronique Trézéguet
May 9, 2009·Biochimica Et Biophysica Acta·Steven M Claypool
Aug 5, 2000·Science·J KaukonenA Suomalainen

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