Probing the role of positive residues in the ADP/ATP carrier from yeast. The effect of six arginine mutations on transport and the four ATP versus ADP exchange modes

Biochemistry
D HeidkämperM Klingenberg

Abstract

Mutagenesis of three intrahelical arginines, R96, R204, or R294, and of each member of the arginine triplet R252, R253, R254 into neutral residues had resulted in a strong suppression of oxidative phosphorylation in cells and isolated mitochondria [Müller, V., Basset, G., Nelson, D. R., & Klingenberg, M. (1996) Biochemistry 35, 16132-16143]. Here we determine the transport activity of wild-type and mutant AAC in reconstituted proteoliposomes using a new rapid removal-stop method without relying on the inhibitor stop which can be compromised by mutations. The basic electroneutral ADP/ADP exchange activity is strongly or totally suppressed in six out of seven of these mutations, with the exception of R294A, which retains nearly wild-type activity. Carboxyatractylate (CAT) inhibits the ADP/ATP exchange rate only to 3-10% in wild type and R294A and up to 40% in other mutants, whereas bongkrekic acid (BKA) inhibits 50% (wild type and R294A) and 90% (other mutants). Consequently, AAC is preferentially reconstituted with the matrix surface outside. All these mutations drastically change activity distribution among the four exchange modes ADP/ADP, ADP/ATP, ATP/ADP, and ATP/ATP. Whereas in wild-type AAC the homo ATP/ATP exchange is twic...Continue Reading

References

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Mar 17, 1975·European Journal of Biochemistry·M KlingenbergB Scherer
Aug 1, 1992·Biochemical Society Transactions·M Klingenberg
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Citations

Sep 22, 2001·Archives of Biochemistry and Biophysics·S G HuangM Klingenberg
Jun 12, 2002·Journal of Molecular and Cellular Cardiology·Carlos ManchadoOctavi Viñas
May 5, 2012·Journal of Bioenergetics and Biomembranes·Gennaro AgrimiFerdinando Palmieri
Oct 12, 2012·Journal of Bioenergetics and Biomembranes·Magnus MonnéGiuseppe Fiermonte
Jan 23, 2009·Nature Cell Biology·Xiaowen WangXin Jie Chen
Feb 3, 2006·Annual Review of Biochemistry·H NuryE Pebay-Peyroula
Aug 24, 2013·Oxidative Medicine and Cellular Longevity·Yaxin Liu, Xin Jie Chen
Feb 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alan J Robinson, Edmund R S Kunji
Sep 10, 2008·The Journal of Cell Biology·Steven M ClaypoolCarla M Koehler
Jul 6, 2010·Current Opinion in Structural Biology·Edmund R S Kunji, Alan J Robinson
May 9, 2009·Biochimica Et Biophysica Acta·Steven M Claypool
Nov 6, 2012·Molecular Membrane Biology·Magnus MonnéEdmund R S Kunji
Nov 24, 2012·Molecular Membrane Biology·John MifsudFrancois Dehez
May 31, 2008·Biochimica Et Biophysica Acta·Martin Klingenberg
Jun 9, 2006·Biochimica Et Biophysica Acta·Edmund R S Kunji, Alan J Robinson
Feb 24, 2010·Biochimica Et Biophysica Acta·Martin Klingenberg
Apr 4, 2003·European Journal of Human Genetics : EJHG·Patrizia De MarcoValeria Capra
Feb 16, 2019·Frontiers in Cell and Developmental Biology·Oluwaseun B Ogunbona, Steven M Claypool
Apr 4, 2021·International Journal of Molecular Sciences·Jürgen KreiterElena E Pohl
Aug 30, 2008·Journal of the American Chemical Society·François DehezChristophe Chipot
Jun 6, 1998·Journal of Molecular Biology·D R NelsonJ M Swanson
Jan 16, 1999·Biochimica Et Biophysica Acta·M Klingenberg, S G Huang

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