Probing the self-assembly and the accompanying structural changes of hydrophobin SC3 on a hydrophobic surface by mass spectrometry

Biophysical Journal
X WangGeorge T Robillard

Abstract

The fungal class I hydrophobin SC3 self-assembles into an amphipathic membrane at hydrophilic-hydrophobic interfaces such as the water-air and water-Teflon interface. During self-assembly, the water-soluble state of SC3 proceeds via the intermediate alpha-helical state to the stable end form called the beta-sheet state. Self-assembly of the hydrophobin at the Teflon surface is arrested in the alpha-helical state. The beta-sheet state can be induced at elevated temperature in the presence of detergent. The structural changes of SC3 were monitored by various mass spectrometry techniques. We show that the so-called second loop of SC3 (C39-S72) has a high affinity for Teflon. Binding of this part of SC3 to Teflon was accompanied by the formation of alpha-helical structure and resulted in low solvent accessibility. The solvent-protected region of the second loop extended upon conversion to the beta-sheet state. In contrast, the C-terminal part of SC3 became more exposed to the solvent. The results indicate that the second loop of class I hydrophobins plays a pivotal role in self-assembly at the hydrophilic-hydrophobic interface. Of interest, this loop is much smaller in case of class II hydrophobins, which may explain the difference...Continue Reading

References

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Citations

Apr 6, 2013·Chemical Reviews·Andreas Walther, Axel H E Müller
Apr 5, 2005·Nature Reviews. Microbiology·Martijn F B G GebbinkHan A B Wösten
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·A H Y KwanJ P Mackay
Jan 27, 2010·Glycobiology·Annunziata ArmenantePaola Giardina
Jun 14, 2012·PLoS Pathogens·Jagadeesh BayryJean-Paul Latgé
Sep 23, 2014·Amino Acids·Baolong NiuWenfeng Li
Sep 19, 2007·Micron : the International Research and Review Journal for Microscopy·Margaret SundeJoel P Mackay
Jul 26, 2015·The Protein Journal·Mohammadreza KhalesiGuy Derdelinckx
Aug 5, 2008·Journal of Molecular Biology·Ann H KwanMargaret Sunde
Jan 8, 2015·Applied Microbiology and Biotechnology·Han A B Wösten, Karin Scholtmeijer
Dec 21, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·S HoumadiE Lacaze

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