Probing the stability of insulin oligomers using electrospray ionization ion mobility mass spectrometry

European Journal of Mass Spectrometry
Uday Kumar Boga RajaLaurence A Angel

Abstract

The peptide hormone insulin is central to regulating carbohydrate and fat metabolism in the body by controlling blood sugar levels. Insulin's most active form is the monomer and the extent of insulin oligomerization is related to insulin's activity of controlling blood sugar levels. Electrospray ionization (ESI) of human insulin produced a series of oligomers from the monomer to the undecamer identified using quadrupole ion mobility time-of-flight mass spectrometry. Previous research suggested that only the monomer, dimer and hexamer are native forms of insulin in solution and the range of oligomers observed in the gas-phase are ESI artifacts. Here the properties of three distinct oligomer bands I, II and III, where both the charge state and number of insulin units of the oligomer increase incrementally, were investigated. When Zn(ii) was added to the insulin sample the same oligomers were observed but with 0-6 Zn(ii) ions bound to each of the oligomers. The oligomers of bands I, II and III were characterized by comparing their drift times, collision cross- sections, relative intensities, collision-induced dissociation (CID) patterns and relative breakdown energies. Insulin oligomers of band I dissociated primarily by releasing...Continue Reading

References

May 13, 1976·Nature·G BentleyD Mercola
Aug 5, 1997·Biochemistry·X ChangJ J Led
Aug 6, 2000·British Journal of Anaesthesia·P Sonksen, J Sonksen
Apr 28, 2001·Critical Reviews in Therapeutic Drug Carrier Systems·M R DeFelippisB H Frank
Sep 15, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Michael F Dunn
Sep 6, 2006·Journal of the American Society for Mass Spectrometry·Raghu K ChittaMichael L Gross
Sep 24, 2008·The Journal of Physical Chemistry. a·P B ArmentroutM T Rodgers
May 9, 2009·European Journal of Mass Spectrometry·David P SmithAlison E Ashcroft
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Virginia G GigantiLaurence A Angel
Mar 16, 2012·Endocrine-related Cancer·Aimee J Varewijck, Joseph A M J L Janssen
Aug 1, 2012·Analytical Chemistry·Matthew F BushCarol V Robinson

❮ Previous
Next ❯

Citations

Oct 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuqing MuVito Ferro
Jul 25, 2019·Journal of the American Society for Mass Spectrometry·Yu-Fu LinLaurence A Angel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.