Probing the structural interactions between methotrexate and dexamethasone with muscle cystatin: a biophysical study.

Journal of Biomolecular Structure & Dynamics
Mohammad AatifBilqees Bano

Abstract

Drug protein interactions have gained considerable attention over the past many years. In the current communication the association of muscle cystatin (MC) with anti-rheumatic drugs methotrexate and dexamethasone was studied by thiol proteinase inhibitor assay, ultra violet (UV) absorption, fluorescence spectroscopy, and fluorescence transform infra-red spectroscopy (FTIR). A static pattern of quenching was noticed between muscle cystatin and methotrexate (MTX). Binding constant (Ka) of methotrexate to muscle cystatin was found to be 1 × 10-7 M-1 and the stoichiometry of binding was calculated to be one. Fluorescence measurement of the emission quenching reveals that the quenching process of cystatin by dexamethasone (DXN) was also static. The stoichiometry of binding and binding constant was also obtained. Additional evidence regarding MTX-MC and DXN-MC was obtained from UV spectroscopy and FTIR spectroscopic results. Such spectroscopic studies would help in modelling new candidate drugs for rheumatoid arthritis based on their cystatin binding profile.Communicated by Ramaswamy H. Sarma.

References

Jun 25, 1987·The New England Journal of Medicine·W T HughesL Lott
Jan 1, 1984·Experimental Cell Biology·P KöppelR Keller
Apr 1, 1996·Molecular and Chemical Neuropathology·H G BernsteinA Rinne
Apr 6, 2002·Expert Opinion on Pharmacotherapy·Barbara BlumenauerPeter Tugwell
Apr 8, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ming GuoQing-Sen Yu
Aug 1, 1990·Parasitology Today·M J NorthG H Coombs
Nov 10, 2004·Journal of Pharmaceutical and Biomedical Analysis·Yan-Jun HuSong-Sheng Qu
Nov 17, 2004·Scandinavian Journal of Gastroenterology·Y MiyazonoT Horie
Jun 8, 2006·Cell Biochemistry and Biophysics·A Ahmed-OuameurH A Tajmir-Riahi
Sep 1, 2006·Cell Biochemistry and Biophysics·J F NeaultH A Tajmir-Riahi
Aug 9, 2007·Journal of Child Psychology and Psychiatry, and Allied Disciplines·Lucie CluverDon Operario
Jan 29, 2008·Colloids and Surfaces. B, Biointerfaces·Mohammad Arif CheemaVíctor Mosquera
Jun 17, 2008·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Shubha PriyamvadaA N K Yusufi
Mar 20, 1947·The Journal of General Physiology·M Kunitz
Feb 6, 2010·Journal of Biochemical and Molecular Toxicology·Soghra Khatun HaqRizwan Hasan Khan
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Graham M WestMichael C Fitzgerald
Nov 26, 2010·European Biophysics Journal : EBJ·Aaliya Shah, Bilqees Bano
Sep 28, 2014·Colloids and Surfaces. B, Biointerfaces·Gulam RabbaniRizwan Hasan Khan
Jan 1, 2009·International Journal of Peptide Research and Therapeutics·Aaliya Shah, Bilqees Bano

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Methods Mentioned

BETA
infrared spectroscopy
fluorescence studies

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