Probing the structure of the mitochondrial channel, VDAC, by site-directed mutagenesis: a progress report.

Journal of Bioenergetics and Biomembranes
E Blachly-DysonM Forte

Abstract

The voltage-dependent anion-selective channel (VDAC) of the mitochondrial outer membrane is formed by a small (approximately 30 kDa) polypeptide, but shares with more complex channels the properties of voltage-dependent gating and ion selectivity. Thus, it is a useful model for studying these properties. The molecular biology techniques available in yeast allow us to construct mutant versions of the cloned yeast VDAC gene in vitro, using oligonucleotide-directed mutagenesis, and to express the mutant genes in yeast cells in the absence of wild-type VDAC. We find that one substitution mutation (lys 61 to glu) alters the selectivity of VDAC.

References

Aug 1, 1987·Journal of Bioenergetics and Biomembranes·M ForteC A Mannella
Aug 1, 1989·Journal of Bioenergetics and Biomembranes·R BenzM Dihanich
Jul 16, 1986·Biochemical and Biophysical Research Communications·D Marguet, G J Lauquin
Dec 21, 1984·Nucleic Acids Research·W KramerH J Fritz
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K MiharaR Sato
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D ShortleD Botstein
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Dec 1, 1985·Plant Physiology·D P Smack, M Colombini

❮ Previous
Next ❯

Citations

Aug 1, 1989·Journal of Bioenergetics and Biomembranes·C A Mannella
Aug 1, 1989·Journal of Bioenergetics and Biomembranes·H TedeschiC A Mannella
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·C A MannellaM Colombini
Feb 15, 1990·Experientia·C A Mannella
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·J Song, M Colombini
Sep 18, 2012·Chemical Reviews·Marco Colombini
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L ThomasM Forte
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel
Aug 4, 2009·Trends in Biochemical Sciences·Marco Colombini
Dec 14, 2011·Biochimica Et Biophysica Acta·Fabrice HombléMartine Prévost
Jan 14, 2012·Biochimica Et Biophysica Acta·Marco Colombini
May 28, 2003·Biochemical and Biophysical Research Communications·Yong ShiHong Tang
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·M C Sorgato, O Moran
Dec 26, 2003·Biophysical Journal·Alexander G KomarovMarco Colombini
Oct 7, 2010·Biophysical Journal·Lamia MlayehFabrice Homblé
Feb 21, 2004·Current Topics in Developmental Biology·Brett H Graham, William J Craigen
Oct 1, 1989·The Journal of Membrane Biology·M Colombini
Oct 1, 1990·Applied Biochemistry and Biotechnology·J S Dordick
Feb 3, 2021·Cell Calcium·William M RosencransTatiana K Rostovtseva

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.