PMID: 9537376Apr 16, 1998Paper

Probing the yeast phase-specific expression of the CBP1 gene in Histoplasma capsulatum

Journal of Bacteriology
J B PatelW E Goldman

Abstract

Histoplasma capsulatum is a pathogenic fungus that exists in two distinct forms. The saprophytic mycelial phase inhabits moist soil environments; once inhaled, hyphae and conidia convert to a unicellular yeast phase that is capable of parasitizing macrophage phagolysosomes. Yeasts cultures, but not mycelial cultures, release large quantities of a calcium-binding protein (CBP) which may be important in calcium acquisition during intracellular parasitism. In this study, we show that the gene encoding CBP (CBP1) is transcriptionally regulated. To identify promoter sequences that are important for yeast phase-specific activity, we created a series of fusions between successively truncated CBP1 5' untranslated regulatory sequences and the Escherichia coli lacZ gene. The fusions were constructed on a telomeric shuttle plasmid that can replicate autonomously in the fungus. By assaying for beta-galactosidase activity from H. capsulatum transformants, we identified a 102-bp region that mediates promoter activation and yeast phase promoter activity. Base pair substitution analysis suggests that the sequences between 839 and 877 bp upstream of the start codon are the most important for this positive regulation.

References

Oct 1, 1991·Clinical Microbiology Reviews·L G Eissenberg, W E Goldman
Jun 1, 1988·Journal of Leukocyte Biology·L G EissenbergW E Goldman
Oct 1, 1988·Molecular & General Genetics : MGG·P L Worsham, W E Goldman
Feb 1, 1994·Journal of Medical Microbiology·L Z GoldaniA M Sugar
Dec 11, 1997·Infection and Immunity·J W Batanghari, W E Goldman
Mar 7, 1998·Molecular Microbiology·J W BatanghariW E Goldman

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Citations

Apr 12, 2005·Eukaryotic Cell·Megan L Bohse, Jon P Woods
Jan 19, 2006·Clinical Microbiology Reviews·Nongnuch VanittanakomThira Sirisanthana
Mar 6, 2007·Eukaryotic Cell·Megan L Bohse, Jon P Woods
Apr 22, 2003·Infection and Immunity·P T MageeDana Davis
Mar 22, 2002·Cellular Microbiology·Peggy J Rooney, Bruce S Klein
Aug 13, 2014·Infection and Immunity·Eric D HolbrookChad A Rappleye
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S KüglerW E Goldman
Jul 1, 2004·Molecular Microbiology·Chad A RappleyeWilliam E Goldman
Feb 18, 2016·Current Genetics·Sean W McCotterJames W Kronstad
Nov 19, 2019·Current Opinion in Microbiology·Anita Sil
Feb 5, 2002·Trends in Microbiology·V Magrini, W E Goldman
Mar 6, 1999·Current Opinion in Microbiology·D R Soll, T Srikantha
Feb 19, 2002·Fungal Genetics and Biology : FG & B·Jon P Woods
Nov 28, 2002·International Journal of Medical Microbiology : IJMM·Atanas Ignatov, Elizabeth J Keath
Nov 19, 2009·BMC Microbiology·Brian H YouseffChad A Rappleye
Aug 14, 1999·Science·W E Goldman

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