Probing viruses with mass spectrometry

Journal of Mass Spectrometry : JMS
G Siuzdak

Abstract

Mass spectrometry offers a new perspective on the solution and gas-phase properties of viruses. Its broad application to local and global viral structure provides unique insights into many biological processes, including viral-antibody binding, protein-protein interactions and protein dynamics. Mass measuring viral proteins is now routine and since viruses are typically well characterized, in that the capsid proteins and DNA (or RNA) sequences are known, identifying a virus based on the mass of the protein and enzymatic digestion fragments is relatively straightforward. Using mass spectrometry, this paper describes the identification of viral protein post-translational modifications such as myristoylation, phosphorylation and disulfide bridging. Furthermore, complementary data obtained with mass spectrometry and x-ray crystallography demonstrate that viruses are highly dynamic particles whose viral capsid's mobility could, until recently, be inferred only from inherently static spectroscopic methods. Lastly, mass spectrometry has been applied on a global scale via the mass measurement of entire intact viruses. Given the general utility of mass spectrometry, its continuing development should further its application to viral dyna...Continue Reading

Citations

Jan 3, 2014·Advances in Virology·Swapnil Subhash BawageShree Ram Singh
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·J K LewisG Siuzdak
Dec 11, 2007·Expert Review of Proteomics·Kasinath Viswanathan, Klaus Früh
Aug 4, 2004·Mass Spectrometry Reviews·Wen-Ping PengHuan-Cheng Chang
May 24, 2005·Rapid Communications in Mass Spectrometry : RCM·Jae-Kuk KimKermit K Murray
Sep 12, 2008·Mass Spectrometry Reviews·Kevin M DownardAlexander B Schwahn
Sep 20, 2012·Drug Testing and Analysis·Nicolas V Schwab, Marcos N Eberlin
Sep 28, 2014·Free Radical Biology & Medicine·Nelmi O Devarie BaezCristina M Furdui
May 29, 2000·Rapid Communications in Mass Spectrometry : RCM·M A StowersC E Kientz
Apr 12, 2002·Mass Spectrometry Reviews·C A Schalley
Jan 7, 2000·Rapid Communications in Mass Spectrometry : RCM·M AdamczykJ Wu
Apr 24, 2002·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·C T ReimannO Tapia
May 2, 2000·The Journal of Biological Chemistry·B BothnerG Siuzdak
Nov 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marco Giampà, Elvira Sgobba
Apr 11, 2000·Accounts of Chemical Research·J J ThomasG Siuzdak
Mar 15, 2000·Journal of Virological Methods·S W TanR M Kini
Feb 12, 2002·Journal of the American Society for Mass Spectrometry·Seetharaman VaidyanathanRoyston Goodacre
Sep 29, 2000·Analytical Chemistry·S D Richardson
Nov 20, 2001·Chemical Reviews·S D Richardson
Jun 29, 1999·Analytical Chemistry·S D Richardson
Nov 20, 2001·Chemical Reviews·S A Hofstadler, R H Griffey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.