Probiotic Bifidobacterium longum alters gut luminal metabolism through modification of the gut microbial community

Scientific Reports
Hirosuke SugaharaHiroshi Ohno

Abstract

Probiotics are well known as health-promoting agents that modulate intestinal microbiota. However, the molecular mechanisms underlying this effect remain unclear. Using gnotobiotic mice harboring 15 strains of predominant human gut-derived microbiota (HGM), we investigated the effects of Bifidobacterium longum BB536 (BB536-HGM) supplementation on the gut luminal metabolism. Nuclear magnetic resonance (NMR)-based metabolomics showed significantly increased fecal levels of pimelate, a precursor of biotin, and butyrate in the BB536-HGM group. In addition, the bioassay revealed significantly elevated fecal levels of biotin in the BB536-HGM group. Metatranscriptomic analysis of fecal microbiota followed by an in vitro bioassay indicated that the elevated biotin level was due to an alteration in metabolism related to biotin synthesis by Bacteroides caccae in this mouse model. Furthermore, the proportion of Eubacterium rectale, a butyrate producer, was significantly higher in the BB536-HGM group than in the group without B. longum BB536 supplementation. Our findings help to elucidate the molecular basis underlying the effect of B. longum BB536 on the gut luminal metabolism through its interactions with the microbial community.

Associated Clinical Trials

Feb 1, 2018·Connie J Rogers

References

Mar 1, 1997·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·M J Hill
Mar 13, 1998·The American Journal of Clinical Nutrition·A Báez-SaldañaE Ortega
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Oct 9, 2002·Genome Research·Dmitry A RodionovMikhail S Gelfand
Aug 29, 2003·Organic Letters·Max J CryleJames J De Voss
Jan 23, 2004·Chemical Communications : Chem Comm·Max J Cryle, James J De Voss
Sep 10, 2004·Plant & Cell Physiology·Jun KikuchiTakashi Hirayama
Nov 13, 2004·The Journal of Nutritional Biochemistry·H SoneY Furukawa
Feb 8, 2006·Current Opinion in Gastroenterology·Hamid M Said, Zainab M Mohammed
Jan 30, 2007·Allergology International : Official Journal of the Japanese Society of Allergology·Jin-zhong XiaoTadao Enomoto
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Oct 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Max J Cryle, Ilme Schlichting
Nov 6, 2008·World Journal of Gastroenterology : WJG·Yue-Xin YangSophie Bourdu-Naturel
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael A MahowaldJeffrey I Gordon
Jul 30, 2009·Environmental Microbiology·Maria L MarcoMichiel Kleerebezem
Oct 13, 2009·Magnetic Resonance in Chemistry : MRC·Ian A LewisJohn L Markley
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jul 28, 2010·Biochemical Society Transactions·Max J Cryle
Aug 10, 2010·Nature Chemical Biology·Steven LinJohn E Cronan
Apr 22, 2011·Nature·Manimozhiyan ArumugamPeer Bork
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Oct 28, 2011·Science Translational Medicine·Nathan P McNultyJeffrey I Gordon
Nov 17, 2011·Nucleic Acids Research·Victor M MarkowitzNikos C Kyrpides
Apr 12, 2012·Nature Reviews. Microbiology·Nicole M KoropatkinEric C Martens
Jun 16, 2012·Nature·Tanya YatsunenkoJeffrey I Gordon
Nov 28, 2012·Current Protocols in Microbiology·Justin KuczynskiRob Knight
Mar 12, 2013·Gut·Mary Ellen SandersEmeran A Mayer
Apr 20, 2013·World Journal of Gastroenterology : WJG·Junko KondoTaeko Shimoda
May 30, 2013·Nucleic Acids Research·Ryan McClureBrian Tjaden
Apr 30, 2014·Immunology and Cell Biology·Kazuhiro IshiguroHidemi Goto
May 23, 2014·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Tamotsu KatoHiroshi Ohno
Sep 12, 2014·Scientific Reports·Patrick VeigaSean P Kennedy

❮ Previous
Next ❯

Citations

Apr 12, 2016·Frontiers in Microbiology·Rebeca MartínPhilippe Langella
Sep 25, 2016·Microbiological Research·Amrita Sarkar, Santanu Mandal
Aug 9, 2016·Molecular Nutrition & Food Research·Borja SánchezAbelardo Margolles
May 16, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lu-Lu WangJian-Dong Jiang
Jul 14, 2018·Journal of Medicinal Food·Hyuk-Jin ChungHyung-Kyoon Choi
Nov 2, 2016·Circulation Research·Monica M SantistebanMohan K Raizada
Jan 19, 2019·Folia Microbiologica·Lucia HrncirovaTomas Hrncir
Mar 17, 2020·Current Opinion in Obstetrics & Gynecology·Sam Schoenmakers, Joop Laven
Jul 22, 2016·PLoS Neglected Tropical Diseases·Victoria Martínez-SernándezFlorencio M Ubeira
Jul 12, 2018·Scientific Reports·Erik DassiNicola Segata
May 7, 2019·Frontiers in Nutrition·Ken YoshiiJun Kunisawa
Aug 2, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Subrata SabuiHamid M Said
Jun 24, 2020·Scientific Reports·Montree Wutthi-InSiriporn Chattipakorn
Oct 25, 2017·The Analyst·Jun Kikuchi, Shunji Yamada
Mar 15, 2019·Microbiology and Molecular Biology Reviews : MMBR·Janneke ElzingaHauke Smidt
Jan 18, 2020·Scientific Reports·Shiro MawatariTakehiko Fujino
Sep 8, 2017·Microbiology and Immunology·Manabu NakanoShouji Hironaka
Dec 6, 2018·International Journal of Molecular Sciences·Lucrecia Carrera-QuintanarRocio I Lopez-Roa
Sep 29, 2017·Frontiers in Microbiology·Chiara FerrarioFrancesca Turroni
Feb 25, 2021·Advances in Nutrition·Despoina Eugenia KiousiAlex Galanis
Feb 7, 2018·Progress in Nuclear Magnetic Resonance Spectroscopy·Jun KikuchiYasuhiro Date

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
PCA
PCR
Assay
RNA-seq
GAM

Software Mentioned

utils
USEARCH
QIIME
Automics
Basic Local Alignment Search Tool ( BLAST )
blastX
BLAST
R
SPSS
CLC Genomics Workbench

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.