Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells.

World Journal of Gastroenterology : WJG
Man-Chin HuaChien-Chang Chen

Abstract

To investigate the immune response of peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) that were stimulated by probiotic preparations. PBMCs were isolated, cultured, and stimulated with Bio-Three (a mixture of Bacillus mesentericus, Clostridium butyricum and Enterococcus faecalis; 10(5), 10(6) and 10(7) CFU/mL for 24 h). Cytokine production of (1) circulating PBMCs; (2) PBMCs stimulated by probiotic preparation; (3) monocyte-derived DCs; and (4) DC and T cell co-culture was determined by enzyme-linked immunosorbent assay. Phenotypic analysis of circulating PBMCs was also investigated by flow cytometry. Blood was obtained from individuals who consumed Bio-Three (10(9) CFU/d B. mesentericus, C. butyricum and E. faecalis) for 2 wk, or those who did not take probiotics orally. In culture supernatants, interferon-gamma (IFN-gamma) and interleukin (IL)-10 production increased, but IL-4 and tumor necrosis factor-alpha (TNF-alpha) production by PBMCs decreased after 1 and 2 wk of probiotic treatment. Flow cytometry was also performed on day 14 and detected enhanced expression of CD11b, HLA-DR, CD4, CD45RA, CD25, CD44 and CD69 in response to Bio-Three. Furthermore, IL-10 and IL-12 were upregulated in supernatants of ...Continue Reading

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Citations

Oct 18, 2013·The Journal of Veterinary Medical Science·Abdul Qadir QadisShigeru Sato
Feb 22, 2012·Beneficial Microbes·J BaderU Stahl
Mar 25, 2020·Journal of Intensive Care Medicine·Suresh Kumar AnguranaSunit Singhi
Feb 2, 2019·Bioscience of Microbiota, Food and Health·Takahiko NagamineMasaru Nakamura
Jun 25, 2020·Vaccines·Georgios Aindelis, Katerina Chlichlia
Mar 7, 2021·International Journal of Molecular Sciences·Kimberley E FreedmanTiffany L Weir
Jun 1, 2021·Frontiers in Immunology·José María Lemme-DumitCarolina Maldonado-Galdeano

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