Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor

Scientific Reports
Mi Ri ParkY Kim

Abstract

Here, we examined the functionality of Lactobacillus fermentum strain JDFM216, a newly isolated probiotic bacterium, using a Caenorhabditis elegans model. We determined bacterial colonization in the intestinal tract of C. elegans by plate counting and transmission electron microscopy and examined the survival of C. elegans using a solid killing assay. In addition, we employed DNA microarray analysis, quantitative real time-polymerase chain reaction, and immunoblotting assays to explore health-promoting pathways induced by probiotic bacteria in C. elegans. Initially, we found that the probiotic bacterium L. fermentum strain JDFM216 was not harmful to the C. elegans host. Conditioning with JDFM216 led to its colonization in the nematode intestine and enhanced resistance in nematodes exposed to food-borne pathogens, including Staphylococcus aureus and Escherichia coli O157:H7. Interestingly, this probiotic strain significantly prolonged the life span of C. elegans. Whole-transcriptome analysis and transgenic worm assays revealed that the health-promoting effects of JDFM216 were mediated by a nuclear hormone receptor (NHR) family and PMK-1 signaling. Taken together, we described a new C. elegans-based system to screen novel probiot...Continue Reading

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Citations

Oct 30, 2019·International Journal of Molecular Sciences·Marianna RoselliChiara Devirgiliis
Mar 14, 2020·Journal of the Science of Food and Agriculture·Irene ZorraquínBegoña Bartolomé
Jul 8, 2020·World Journal of Microbiology & Biotechnology·Maria MarsovaValeriy Danilenko
Dec 31, 2020·Critical Reviews in Food Science and Nutrition·Zongxin LingShaochang Wu
Jan 12, 2021·Frontiers in Nutrition·Cyril PoupetStéphanie Bornes
Mar 26, 2021·Frontiers in Immunology·Mengzhou ZhouJoshua Gong
Apr 4, 2021·Microorganisms·Harish NarasimhanKristyn E Sylvia
May 4, 2021·Frontiers in Microbiology·Diego Francisco Benítez-ChaoJosé Rubén Morones-Ramírez
May 22, 2021·Food Science of Animal Resources·Hayoung KimYounghoon Kim
Jun 23, 2021·Journal of Applied Microbiology·R KatayamaE Kage-Nakadai
Nov 22, 2019·Current Clinical Pharmacology·Kulapong Jayanama, Olga Theou
Aug 19, 2021·Journal of Agricultural and Food Chemistry·Minhye ShinYounghoon Kim
Oct 28, 2021·Microbiology Spectrum·Ayano TsuruEriko Kage-Nakadai

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Methods Mentioned

BETA
transmission electron microscopy
dissection
chip
chips
PCR

Software Mentioned

Primer3Input
GeneSpring GX
GeneSpring
LOWESS

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