Probiotic Lactobacillus Paracasei Expressing a Nucleic Acid-Hydrolyzing Minibody (3D8 Scfv) Enhances Probiotic Activities in Mice Intestine as Revealed by Metagenomic Analyses

Genes
Seungchan ChoSukchan Lee

Abstract

Probiotics are well known for their beneficial effects for animals, including humans and livestock. Here, we tested the probiotic activity of Lactobacillus paracasei expressing 3D8 scFv, a nucleic acid-hydrolyzing mini-antibody, in mice intestine. A total of 18 fecal samples derived from three different conditions at two different time points were subjected to high-throughput 16S ribosomal RNA (rRNA) metagenomic analyses. Bioinformatic analyses identified an average of 290 operational taxonomic units. After administration of L. paracasei, populations of the probiotics L. paracasei, Lactobacillus reuteri, and Pediococcus acidilactici increased, whereas the population of harmful bacteria such as Helicobacter species decreased. Furthermore, continuous administration of L. paracasei resulted in L. paracasei emerging as the dominant probiotic after competition with other existing probiotics. Expression of 3D8 scFv protein specifically increased the population of P. acidilactici, which is another probiotic. In summary, our results showed that L. paracasei expressing 3D8 scFv protein enhanced probiotic activity in mice intestine with no observable side effects. Thus, the system developed in this study may be a good tool for the expres...Continue Reading

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Citations

Dec 12, 2018·Microbiology Resource Announcements·Dongjun KimSukchan Lee
Sep 6, 2019·Helicobacter·Armelle Ménard, Annemieke Smet

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Datasets Mentioned

BETA
PRJNA352304

Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

CD
STAMP
Illumina package
MiSeq Reporter ( MSR )
MiSeq Control Software ( MCS
Real Time Analysis ( RTA
HIT
Excel Data Analysis
QIIME
OUT

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