Probiotic metabolites from Bacillus coagulans GanedenBC30™ support maturation of antigen-presenting cells in vitro.

World Journal of Gastroenterology : WJG
Kathleen F BensonGitte S Jensen

Abstract

To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells. Ganeden Bacillus coagulans 30 (GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite (MET) fraction. A second fraction was made to generate a crude cell-wall-enriched fraction, by centrifugation and lysis, followed by washing. A preparation of MET was subjected to size exclusion centrifugation, generating three fractions: < 3 kDa, 3-30 kDa, and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell (PBMC) as a source of antigen-presenting mononuclear phagocytes. The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14, CD16, CD80 and CD86 and analyzed by flow cytometry. Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes. The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells, and this property was associated with the high molecular weight metabolite fraction. Changes were also seen for the dendritic cell maturation markers CD80 a...Continue Reading

References

Jan 1, 1977·Annual Review of Microbiology·D C Savage
Dec 29, 2000·Nature·H HemmiS Akira
Dec 1, 2006·Journal of Leukocyte Biology·Loems Ziegler-Heitbrock
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel N FrankNorman R Pace
May 30, 2008·Nature·Sarkis K MazmanianDennis L Kasper
Dec 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sergey R KonstantinovYvette van Kooyk
Mar 3, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J R EndresA G Schauss
Oct 13, 2009·Cellular Immunology·P KvistborgM B Zocca
Dec 29, 2009·Frontiers in Bioscience (Landmark Edition)·Erin B Troy, Dennis L Kasper
Mar 6, 2010·Science·Matam Vijay-KumarAndrew T Gewirtz
May 22, 2010·Science·UNKNOWN Human Microbiome Jumpstart Reference Strains ConsortiumDianhui Zhu
Jul 14, 2010·Nature Reviews. Microbiology·Sandra NellChristine Josenhans
Jul 16, 2010·Blood·Loems Ziegler-HeitbrockManfred B Lutz
Jun 11, 2011·Journal of Biomedicine & Biotechnology·Valeria RizzelloGuido Ferlazzo

❮ Previous
Next ❯

Citations

May 27, 2014·International Journal of Environmental Research and Public Health·Sabina Fijan
Mar 10, 2016·Applied Microbiology and Biotechnology·Elisa SalvettiGiuseppe Spano
Aug 12, 2014·Journal of Medicinal Food·Gitte S JensenKathleen F Benson
Jul 10, 2019·Journal of Clinical Medicine·Young Bok LeeHei Sung Kim
Sep 10, 2013·Probiotics and Antimicrobial Proteins·Jeffrey A Banas, Eric T Popp
Jun 15, 2018·Foods·Gözde Konuray, Zerrin Erginkaya
Mar 19, 2020·Animals : an Open Access Journal From MDPI·Yuanhao ZhouWeifen Li
Jun 27, 2013·Current Opinion in Clinical Nutrition and Metabolic Care·Sue-Joan Chang, Hsiu-Hua Huang
Nov 1, 2013·Comprehensive Reviews in Food Science and Food Safety·Rocío I Corona-HernandezAbraham Wall-Medrano
Mar 5, 2021·Life Sciences·Arpita AroraSimona Bungau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.