Probiotic mixture of Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 attenuates hippocampal apoptosis induced by lipopolysaccharide in rats

International Microbiology : the Official Journal of the Spanish Society for Microbiology
Ghazaleh MohammadiMarjan Nassiri-Asl

Abstract

In recent years, the beneficial impact of targeted gut microbiota manipulation in various neurological disorders has become more evident. Therefore, probiotics have been considered as a promising approach to modulate brain gene expression and neuronal pathways even in some neurodegenerative diseases. The purpose of this study was to determine the effect of probiotic biotherapy with combination of Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 on the expression levels of proteins critical to neuronal apoptosis in hippocampus of lipopolysaccharide (LPS)-exposed rats. Four groups of animals (Control, LPS, Probiotic + LPS, and Probiotic) were treated with maltodextrin (placebo) or probiotic (109 CFU/ml/rat) for 2 weeks by gavage. On the 15th day, a single intraperitoneal dose of saline or LPS (1 mg/kg) was injected and 4 h later, protein assessment was performed by western blotting in hippocampal tissues. LPS significantly increased the Bax, Bax/Bcl-2 ratio, and cleaved caspase-3 expression along with decreased the Bcl-2 and procaspase-3 protein levels. However, probiotic pretreatment (L. helveticus R0052 + B. longum R0175) significantly downregulated the Bax and Bax/Bcl-2 ratio accompanied with upregulated Bcl-2 e...Continue Reading

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Citations

Sep 25, 2020·The FEBS Journal·Laura BonfiliAnna Maria Eleuteri
May 1, 2021·Pharmaceuticals·Oliwia Gawlik-Kotelnicka, Dominik Strzelecki
Jul 21, 2021·Cellular and Molecular Neurobiology·Hamed MirzaeiOmid Reza Tamtaji
Oct 12, 2021·Journal of Agricultural and Food Chemistry·Jiaming LiuJing Sun

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Methods Mentioned

BETA
Protein Assay
electrophoresis

Software Mentioned

GraphPad Prism
Image J

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis