Probiotics in human gut microbiota can degrade host glycosaminoglycans

Scientific Reports
Keigo KawaiWataru Hashimoto

Abstract

Glycosaminoglycans (GAGs) (e.g. heparin, chondroitin sulfate, and hyaluronan) show various significant physiological functions as a major component of extracellular matrix in animals. Some bacteria target GAGs for adhesion and/or infection to host cells, although no probiotics have been known to degrade GAGs. Here, we show GAG degradation by probiotics from human gut microbiota and their adhesion to human intestinal cells through a GAG. GAG-degrading bacteria were isolated from human faeces and identified as Enterococcus faecium, and some typical probiotics such as Lactobacillus casei, Lactobacillus rhamnosus and Enterococcus faecalis were also found to degrade heparin. GAG-degrading lactobacilli and enterococci including the isolated E. faecium possessed a genetic cluster encoding GAG-degrading/metabolising enzymes in the bacterial genome. KduI and KduD enzymes encoded in the GAG cluster of L. rhamnosus functioned as 4-deoxy-l-threo-5-hexosulose-uronate ketol-isomerase and 2-keto-3-deoxy-d-gluconate dehydrogenase, respectively, both of which were crucial for GAG metabolism. GAG-degrading L. rhamnosus and E. faecium attached to human intestinal Caco-2 cells via heparin. Some species of Bacteroides, considered to be the next gen...Continue Reading

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Apr 23, 2017·Scientific Reports·Sayoko OikiWataru Hashimoto

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Citations

Nov 7, 2019·Food Science and Biotechnology·Na-Kyoung LeeHyun-Dong Paik
Nov 17, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hai-Yu ZhangXiao-Lin Tong
Mar 23, 2021·Frontiers in Microbiology·Eric Banan-Mwine DaliriDeog-Hwan Oh
Jun 5, 2021·Frontiers in Cellular and Infection Microbiology·Virginia Perez-CarrascoJose A Garcia-Salcedo
Jul 14, 2021·Scientific Reports·Vorthon SawaswongSunchai Payungporn
Sep 7, 2021·Frontiers in Microbiology·Maia Segura-WangMahdi Ghanbari
Apr 8, 2019·Journal of Ethnopharmacology·Zhiwen ZhangZhenghong Xu

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Datasets Mentioned

BETA
BEHC01000001-BEHC01000108

Methods Mentioned

BETA
phosphotransferase
competition binding
PCR

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