Probiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month's storage on the International Space Station

Scientific Reports
Takafumi SakaiSatoshi Furukawa

Abstract

Suppression of immune function during long spaceflights is an issue that needs to be overcome. The well-established probiotic Lactobacillus casei strain Shirota (LcS) could be a promising countermeasure, and we have launched a project to investigate the efficacy of its use on the International Space Station (ISS). As a first step, we developed a specialist probiotic product for space experiments, containing freeze-dried LcS in capsule form (Probiotics Package), and tested its stability through 1 month of storage on the ISS. The temperature inside the ISS ranged from 20.0 to 24.5 °C. The absorbed dose rate of the flight sample was 0.26 mGy/day and the dose equivalent rate was 0.52 mSv/day. The number of live LcS was 1.05 × 1011 colony-forming units/g powder (49.5% of the initial value) 6 months after the start of the study; this value was comparable to those in the two ground controls. Profiles of randomly amplified polymorphic DNA, sequence variant frequency, carbohydrate fermentation, reactivity to LcS-specific antibody, and the cytokine-inducing ability of LcS in the flight sample did not differ from those of the ground controls. We can therefore maintain the viability and basic probiotic properties of LcS stored as a Probiot...Continue Reading

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Citations

Sep 7, 2019·Nature Communications·Maximilian MoraChristine Moissl-Eichinger
Sep 29, 2019·The New Bioethics : a Multidisciplinary Journal of Biotechnology and the Body·Konrad Szocik, Martin Braddock
Apr 28, 2021·Probiotics and Antimicrobial Proteins·K S YohaC Anandharamakrishnan

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Methods Mentioned

BETA
ISS
PCR
genetic modification
PMA
electrophoresis
enzyme-linked immunosorbent assay

Software Mentioned

Bio PADLES package ( JAXA )
Bio PADLES
CLC Genomics Workbench
JAXA

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