PMID: 7315842Nov 1, 1981

Problems in measurement of erythrocyte calcium

American Journal of Hematology
E A O'RearE C Lynch

Abstract

As calcium has increasingly been the object of study in erythrocyte physiology, we reviewed the current methodologies for determination of calcium by atomic absorption spectrometry. The published normal values for erythrocyte calcium vary from 5 to 500 mumol/liter of packed cells. A method based on Harrison and Long's determination of calcium is presented and shows normal red cell calcium concentration to be 0.0149 +/- 0.0023 mumol/ml of packed red cells. The influence of temperature and type of crucible used in ashing red cells is assessed. The method of additions is employed to corroborate our results.

References

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Jan 31, 1978·Pflügers Archiv : European journal of physiology·H Rogausch
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Citations

Jan 1, 1987·The Journal of Membrane Biology·B Engelmann, J Duhm
Oct 1, 1994·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M Kurata, M Suzuki
Nov 1, 1996·Clinical and Experimental Hypertension : CHE·M A NewazN N Htut
Nov 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Lucile VincentLaurent Messonnier

Related Concepts

Calcium
Erythrocytes
Hydrogen Chloride
Orthophosphate
Sodium Hydroxide
Spectrophotometry, Atomic

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