Probucol enhances the therapeutic efficiency of mesenchymal stem cells in the treatment of erectile dysfunction in diabetic rats by prolonging their survival time via Nrf2 pathway.

Stem Cell Research & Therapy
Haoran WangQiang Fu

Abstract

Intracavernous injection of mesenchymal stem cells (MSCs) is a promising method for diabetic mellitus-induced erectile dysfunction (DMED), but short survival time of MSCs in cavernous is a fatal defect for therapy. This study investigated therapeutic efficiency and potential mechanism of probucol combined with MSCs. In vivo study, a total of forty-eight 10-week-old male Sprague-Dawley (SD) rats were used. Twelve rats received intraperitoneal injection of PBS as the sham group; the rest received intraperitoneal injection of 60 mg/kg streptozotocin to establish DM models. DM rats were randomly divided into three groups: received intracavernosal (IC) injection of either PBS (DM group), MSCs (M group), or administrated probucol after intracavernosal injection of MSCs (P + M group). Erectile function was assessed by electrical stimulation of the cavernous nerves with real-time intracavernous pressure measurement. After euthanasia, penile tissue was investigated for histologic examination and Western blotting. In in vitro experiment, H2O2 was used to create oxidative stress environment to detect changes in cell viability. CCK8 was used to measure cell viability of MSCs treated with or without probucol. Intracellular ROS changes were ...Continue Reading

References

Nov 22, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Nobuo N NodaFuyuhiko Inagaki
Sep 22, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Derek A Drechsel, Manisha Patel
Jan 26, 2012·The Journal of Biological Chemistry·Suryakant K Niture, Anil K Jaiswal
Jan 30, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Takuya NaritaKen Suzuki
Oct 14, 2014·Methods in Molecular Biology·Gaurav S ChoudharyAlexandru Almasan
Feb 28, 2015·Cellular and Molecular Neurobiology·Lulu LiQiang Zhang
Mar 11, 2015·Stem Cell Research·Ben MeadBen A Scheven
Apr 29, 2015·The Journal of Endocrinology·Bo ChenJingqiu Cheng
Jul 19, 2016·Autophagy·Marta PajaresAntonio Cuadrado
May 26, 2017·Cellular & Molecular Biology Letters·Wei Jing LiuHua Feng Liu
Jul 20, 2017·Diabetic Medicine : a Journal of the British Diabetic Association·Y KouidratN Veronese
Sep 14, 2017·Antioxidants & Redox Signaling·Claudia TonelliDavid A Tuveson
Sep 20, 2017·Sexual Medicine Reviews·Faysal A YafiEdgardo F Becher
Oct 23, 2017·European Journal of Pharmacology·Minli ZhangDaqing Zhang
Apr 11, 2018·Sexual Medicine Reviews·Ethan L MatzAnthony Atala
Jul 28, 2018·International Journal of Impotence Research·I MoncadaJ Romero
Aug 30, 2018·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Hongbin ZhangZhong Zeng
Apr 26, 2019·Human & Experimental Toxicology·L A Méndez-GarcíaE J Córdova
Apr 30, 2019·Primary Care·Gretchen M Irwin
May 3, 2019·The New England Journal of Medicine·Helen M Blau, George Q Daley

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence-activated cell sorting
electrophoresis
confocal microscopy
flow cytometry
Fluorescence microscopy

Software Mentioned

SPSS
Multigauge

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis