Procedure for isolating the endophyte from tall fescue and screening isolates for ergot alkaloids.

Applied and Environmental Microbiology
C W Bacon

Abstract

A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolated mycelium and characteristic conidia within a 3- to 3.5-week period. Once isolated, each fungus was placed in another liquid medium, M104T, where competent strains produced total ergot alkaloids ranging from 38 to 797 mg/liter. Several isolates were negative for ergot alkaloid synthesis. The production of ergot alkaloids by individual isolates was unstable; isolates rapidly degenerated in their ability to produce ergot alkaloids during subculture. However, the procedure as presented allows the assessment of an isolate for ergot alkaloid synthesis during its initial isolation.

References

May 1, 1979·Journal of Agricultural and Food Chemistry·J K PorterJ D Robbins
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Citations

Jan 1, 1997·Folia Microbiologica·M FliegerR Shelby
Jan 25, 2014·Mycologia·Adrian LeuchtmannMariusz Tadych
Apr 16, 2015·Toxins·Carolyn A YoungJolanta Jaromczyk
Aug 4, 2015·Current Protocols in Microbiology·Simona FloreaWalter Hollin
Dec 1, 1990·Journal of Chemical Ecology·M R SiegelM C Johnson
Apr 2, 2015·Preparative Biochemistry & Biotechnology·Prajna Rao Krishnapura, Prasanna D Belur
Nov 20, 2014·Frontiers in Chemistry·Carolyn A YoungAndrew A Hopkins
Nov 1, 1993·Applied and Environmental Microbiology·N H FergusonN G Allgood
Apr 1, 1995·Applied and Environmental Microbiology·O J BallJ M Sprosen
Oct 29, 2000·Journal of Agricultural and Food Chemistry·Q YueM D Richardson

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