PROCEED: A proteomic method for analysing plasma membrane proteins in living mammalian cells

Briefings in Functional Genomics & Proteomics
Yaniv BlediMichal Linial

Abstract

Elucidating the profile of extracellular integral membrane proteins on live cells is vital for uncovering diagnostic disease biomarkers, therapeutic agents and drug receptor candidates. Exploring the realm of these proteins has proved to be an intricate task, mainly due to their hydrophobic nature and low abundance. Furthermore, the level of purity achieved by classical methods of purification and cell fractionation is insufficient. These restrictions pose major limitations for gel electrophoresis or chromatography-based separation techniques as the preferred methodologies for high-throughput analysis. Mass spectrometry has alleviated most of the difficulties in the identification of proteins in general; however, the Achilles' heel is still the isolation and separation of membrane proteins. In order to circumvent these limitations, a high-throughput platform has been devised, whereby proteases are applied to whole intact living cells. The resulting peptide fragments are then analysed by liquid chromatology followed by tandem MS (LC-MS/MS) technology to provide a detailed profile of proteins exposed on the surface of the plasma membrane. This kind of protein trimming offers the advantages that no prior manipulation or fractionat...Continue Reading

Citations

May 31, 2008·Briefings in Functional Genomics & Proteomics·Daniel Martins de SouzaJosé C Novello
Jun 10, 2014·International Reviews of Immunology·Leonardo Chicaybam, Martin Hernan Bonamino
Sep 4, 2008·Proteomics·Sandra TanMaxey C M Chung
Jul 15, 2015·Proteomics·Sandra BurghoffJürgen Schrader
Mar 21, 2006·Journal of Chromatography. a·Marijana RucevicDjuro Josic
Sep 19, 2007·Molecular & Cellular Proteomics : MCP·Prabodhika MallikaratchyWeihong Tan
Jul 27, 2007·Proteomics·Djuro Josic, James G Clifton
Sep 10, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Rolf ApweilerGerd Schmitz
Dec 13, 2016·BMC Biotechnology·Anam Qudrat, Kevin Truong
Mar 4, 2021·Mass Spectrometry Reviews·Jarne PauwelsKris Gevaert
Apr 13, 2005·Journal of Proteome Research·Aly KarsanTimothy Veenstra
Aug 9, 2007·Chemical Reviews·Anna E Speers, Christine C Wu
Aug 7, 2007·Journal of Proteome Research·Jun LiuJing Zhang
Jun 3, 2006·Journal of Proteome Research·James MartosellaBarry E Boyes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.