Process of tight junction recovery in the injured vocal fold epithelium: Morphological and paracellular permeability analysis

The Laryngoscope
Ryo SuzukiKoichi Omori

Abstract

The vocal fold epithelium that includes tight junction (TJ)-based barrier function protects underlying connective tissues from external insults. TJs play an important role to control paracellular permeability of not only solutes but also ions, and preserve the vocal fold homeostasis. However, the distribution of TJs and paracellular diffusion barrier across the entire vocal fold epithelium are still unknown. The aim of this study was to identify the distribution of TJs in the vocal fold epithelium and to characterize the recovery process of TJ-based paracellular diffusion barrier in a rat model of vocal fold injury. Animal experiments with controls. Normal and vocal fold-injured rats were used. Larynges were harvested for immunohistochemical examination of TJ proteins. For functional analysis, a tracer permeability assay was performed using EZ-Link Sulfo-NHS-LC-Biotin. TJ proteins occludin and zonula occludens 1 signals were localized to the junctional regions of the most luminal cell layers of the vocal fold epithelium. The injured region had been recovered with epithelium at 5 days postinjury, but the paracellular diffusion barrier assays revealed that biotinylation reagents diffused into the lamina propria at 5 days postinju...Continue Reading

References

May 1, 1988·Acta Oto-laryngologica·M N KotbyM M Saleh
Jan 1, 1994·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·T ShinI Nahm
Dec 1, 1993·The Journal of Cell Biology·M FuruseS Tsukita
Jan 1, 1993·Acta Oto-laryngologica·F G DikkersF J Cervera-Paz
Apr 29, 1998·Developmental Biology·C S MerzdorfD A Goodenough
Apr 29, 1998·Annual Review of Physiology·L L Mitic, J M Anderson
Dec 5, 2000·Molecular Biology of the Cell·M SaitouS Tsukita
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·S TsukitaM Itoh
May 11, 2002·Journal of Voice : Official Journal of the Voice Foundation·Susan L ThibeaultCharles N Ford
Aug 23, 2002·Trends in Cell Biology·Daisuke TsurutaJonathan C R Jones
May 14, 2003·The Journal of Cell Biology·Takehiro NittaShoichiro Tsukita
Jun 5, 2003·The Laryngoscope·Shigeru HiranoCharles N Ford
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·Karl Matter, Maria S Balda
May 1, 1963·The Journal of Cell Biology·M G FARQUHAR, G E PALADE
May 21, 2004·American Journal of Physiology. Cell Physiology·Eveline E Schneeberger, Robert D Lynch
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Hongyu LiMartin J Hug
Nov 13, 2004·The Annals of Otology, Rhinology, and Laryngology·Bernard RousseauDiane M Bless
Apr 14, 2005·The Annals of Otology, Rhinology, and Laryngology·Tomoko TateyaDiane M Bless
Feb 8, 2006·Annual Review of Physiology·Christina M Van Itallie, James M Anderson
Mar 16, 2006·Trends in Cell Biology·Mikio Furuse, Shoichiro Tsukita
May 9, 2006·The Annals of Otology, Rhinology, and Laryngology·Tomoko TateyaDiane M Bless
Jun 15, 2006·Annual Review of Cell and Developmental Biology·Kunyoo ShinBen Margolis
Sep 15, 2007·Biochimica Et Biophysica Acta·Andrea Hartsock, W James Nelson
May 16, 2008·American Journal of Physiology. Renal Physiology·Susanne AngelowAlan S L Yu
Sep 3, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Christine PohlCharles James Kirkpatrick
Jun 3, 2009·Brazilian Journal of Otorhinolaryngology·Regina Helena Garcia MartinsJosé Reinaldo Cerqueira Braz
Dec 10, 2009·The Journal of Experimental Medicine·Akiharu KuboMasayuki Amagai
Jan 26, 2010·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Mahalakshmi SivasankarRyan C Branski
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Mikio Furuse
Jun 22, 2010·The Laryngoscope·Elizabeth Erickson, Mahalakshmi Sivasankar
Jan 29, 2011·The Laryngoscope·Bernard RousseauMahalakshmi Sivasankar
Jul 1, 2011·Methods in Molecular Biology·Lei DingYan-Hua Chen
Sep 8, 2011·The Laryngoscope·Rebecca AlperMahalakshmi Sivasankar
Apr 17, 2013·Physiological Reviews·Dorothee Günzel, Alan S L Yu
Apr 2, 2014·Journal of Speech, Language, and Hearing Research : JSLHR·Elizabeth Erickson LevendoskiSusan L Thibeault
Jul 22, 2014·The Laryngoscope·Ciara LeydonMarvin P Fried

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.