Process optimization for the production of diosgenin with Trichoderma reesei

Bioprocess and Biosystems Engineering
Yuling ZhuWen Huang

Abstract

Based on the response surface methodology, an effective microbial system for diosgenin production from enzymatic pretreated Dioscorea zingiberensis tubers with Trichoderma reesei was studied. The fermentation medium was optimized with central composite design (3(5)) depended on Plackett-Burmann design which identified significant impacts of peptone, K(2)HPO(4) and Tween 80 on diosgenin yield. The effects of different fermentation conditions on diosgenin production were also studied. Four parameters, i.e. incubation period, temperature, initial pH and substrate concentration were optimized using 4(5) central composite design. The highest diosgenin yield of 90.57% was achieved with 2.67% (w/v) of peptone, 0.29% (w/v) of K(2)HPO(4), 0.73% (w/v) of Tween 80 and 9.77% (w/v) of substrate, under the condition of pH 5.8, temperature 30 degrees C. The idealized incubation time was 6.5 days. After optimization, the product yield increased by 33.70% as compared to 67.74 +/- 1.54% of diosgenin yield in not optimized condition. Scale-up fermentation was carried out in a 5.0 l bioreactor, maximum diosgenin yield of 90.17 +/- 3.12% was obtained at an aeration of 0.80 vvm and an agitation rate of 300 rpm. The proposed microbial system is clean...Continue Reading

References

Sep 1, 1979·Journal of Bacteriology·D Sternberg, G R Mandels
May 1, 1993·Biochemical Society Transactions·R E HollandD Billington
Jan 7, 1993·Biochemical Pharmacology·R E HollandD Billington
Jun 13, 2002·Bioresource Technology·Ye Sun, Jiayang Cheng
Oct 20, 2004·Bioresource Technology·Zhiyou WenShulin Chen
Nov 8, 2007·Bioprocess and Biosystems Engineering·Manav B BeraBahadur Singh
May 16, 2008·Bioprocess and Biosystems Engineering·Ming-Jie JinChao Peng
Jun 3, 2008·Bioresource Technology·Mani Shankar BhattacharyyaUttam Chand Banerjee

❮ Previous
Next ❯

Citations

Jul 19, 2013·World Journal of Microbiology & Biotechnology·Yuling ZhuJinren Ni
Feb 14, 2017·Applied Microbiology and Biotechnology·Dorothee Barth, Marilyn G Wiebe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.