PMID: 2117827Sep 1, 1990Paper

Processing and intracellular transport of rubella virus structural proteins in COS cells

Virology
T C HobmanS Gillam

Abstract

Plasmids encoding rubella virus (RV) structural proteins C-E2-E1, E2-E1, E2, and E1 have been constructed in the eukaryotic expression vector pCMV5. The processing and intracellular transport of these proteins have been examined by transient expression of the cDNAs in COS cells. Compared to alphaviruses, processing of RV glycoprotein moieties occurred relatively slowly and the transport of glycoproteins E2 and E1 to the plasma membrane was inefficient. Indirect immunofluorescence revealed that the majority of RV antigen in transfected and infected COS cells was localized to the Golgi region, including the capsid protein. Accumulation of capsid protein in the juxtanuclear region was determined to be RV glycoprotein dependent. Unlike alphaviruses, RV E1 did not require E2 for targeting to the Golgi where it was retained. E2 was however necessary for cell surface expression of E1. This study revealed that the processing and transport of RV structural proteins is quite different from alphaviruses and that the accumulation of antigens in the Golgi region may be significant in light of previous reports which suggest that RV buds from the internal membranes in some cell types.

References

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Citations

Oct 1, 1994·Journal of Medical Virology·N O Seto, S Gillam
Mar 1, 1993·Journal of Clinical Immunology·H ChayeS Gillam
Jul 1, 1993·Trends in Microbiology·T C Hobman
Aug 1, 1992·The Journal of Cell Biology·T C HobmanM G Farquhar
Apr 1, 1993·The Journal of Cell Biology·T C HobmanM G Farquhar
Oct 12, 2000·Clinical Microbiology Reviews·J Y Lee, D S Bowden
Feb 29, 2008·Journal of Virology·Carolina S IlkowTom C Hobman
Jun 16, 2005·Intervirology·Hongling Wen, Zhiyu Wang
Dec 22, 1999·Virology·J Y LeeD S Bowden
Apr 10, 1999·Journal of Virology·M GarbuttT C Hobman
Feb 26, 2021·Journal of Virology·Pratyush Kumar Das, Margaret Kielian
Sep 1, 1992·Journal of Clinical Microbiology·H H ChayeS Gillam
Mar 1, 1996·Clinical and Diagnostic Laboratory Immunology·M SchmidtC Oker-Blom
Sep 1, 1991·Journal of Clinical Microbiology·H SeppänenC Oker-Blom

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