PMID: 8985386Jan 1, 1997Paper

Processing of a cellular polypeptide by 3CD proteinase is required for poliovirus ribonucleoprotein complex formation

Journal of Virology
Holger H RoehlBert L Semler

Abstract

Poliovirus interactions with host cells were investigated by studying the formation of ribonucleoprotein complexes at the 3' end of poliovirus negative-strand RNA which are presumed to be involved in viral RNA synthesis. It was previously shown that two host cell proteins with molecular masses of 36 and 38 kDa bind to the 3' end of viral negative-strand RNA at approximately 3 to 4 h after infection. We tested the hypothesis that preexisting cellular proteins are modified during the course of infection and are subsequently recruited to play a role in viral replication. It was demonstrated that the 38-kDa protein, either directly or indirectly, is the product of processing by poliovirus 3CD/3C proteinase. Only the modified 38-kDa protein, not its precursor protein, has a high affinity for binding to the 3' end of viral negative-strand RNA. This modification depends on proteolytically active proteinase, and a direct correlation between the levels of 3CD proteinase and the 38-kDa protein was demonstrated in infected tissue culture cells. The nucleotide (nt) 5-10 region (positive-strand numbers) of poliovirus negative-strand RNA is important for binding of the 38-kDa protein. Deletion of the nt 5-10 region in full-length, positive-s...Continue Reading

References

Aug 1, 1992·Journal of Virology·A A Haller, B L Semler
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May 1, 1991·Journal of Virology·W A ChariniB L Semler
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Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·L B BlynE Ehrenfeld

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Citations

Sep 29, 1999·The Journal of General Virology·R ZellA Stelzner
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·D A MatthewsS T Worland
Jan 15, 2004·Journal of Virology·David M BrownBert L Semler
Aug 17, 2004·Journal of Virology·Rajeev BanerjeeAsim Dasgupta
Jun 15, 2007·Journal of Virology·George A BelovEllie Ehrenfeld
May 26, 2010·Cellular Microbiology·George A BelovEllie Ehrenfeld
Apr 18, 1998·The Journal of Biological Chemistry·M T Beckman, K Kirkegaard
Aug 16, 2019·Journal of Virology·Autumn C Holmes, Bert L Semler
Mar 12, 2021·Journal of Cell Science·Jiamin YiHaixue Zheng
Nov 5, 1997·Archives of Biochemistry and Biophysics·G J DavisE C Villarreal
Oct 3, 1999·Virus Research·V I AgolE Wimmer
Sep 29, 2009·Biochimica Et Biophysica Acta·Ying LiuAniko V Paul
Aug 8, 1998·Genes & Development·A V Gamarnik, R Andino

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