Processing of a dicistronic small nucleolar RNA precursor by the RNA endonuclease Rnt1

The EMBO Journal
Guillaume ChanfreauA Jacquier

Abstract

Small nucleolar RNAs (snoRNAs) are intron encoded or expressed from monocistronic independent transcription units, or, in the case of plants, from polycistronic clusters. We show that the snR190 and U14 snoRNAs from the yeast Saccharomyces cerevisiae are co-transcribed as a dicistronic precursor which is processed by the RNA endonuclease Rnt1, the yeast ortholog of bacterial RNase III. RNT1 disruption results in a dramatic decrease in the levels of mature U14 and snR190 and in accumulation of dicistronic snR190-U14 RNAs. Addition of recombinant Rnt1 to yeast extracts made from RNT1 disruptants induces the chase of dicistronic RNAs into mature snoRNAs, showing that dicistronic RNAs correspond to functional precursors stalled in the processing pathway. Rnt1 cleaves a dicistronic transcript in vitro in the absence of other factors, separating snR190 from U14. Thus, one of the functions of eukaryotic RNase III is, as for the bacterial enzyme, to liberate monocistronic RNAs from polycistronic transcripts.

References

Nov 25, 1990·Nucleic Acids Research·J Liu, E S Maxwell
Jan 1, 1994·Methods in Molecular Biology·M Zuker
Apr 1, 1995·Genes & Development·J G Umen, C Guthrie
Oct 1, 1995·Genes & Development·W Q Liang, M J Fournier
Jan 1, 1995·Annual Review of Biochemistry·E S Maxwell, M J Fournier
Jul 1, 1995·Trends in Biochemical Sciences·J P BachellerieM J Fournier
Jun 1, 1995·Genes & Development·T Kiss, W Filipowicz
Jul 27, 1993·Biochemistry·B ChelladuraiA W Nicholson
Jun 15, 1996·Nucleic Acids Research·G Rotondo, D Frendewey
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D Lafontaine, D Tollervey
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·K T TycowskiJ A Steitz
Jun 1, 1997·Current Opinion in Cell Biology·D Tollervey, T Kiss
May 30, 1997·Cell·C M Smith, J A Steitz
Jun 1, 1997·Chromosoma·J P Morrissey, D Tollervey
Oct 23, 1997·Genes & Development·G ChanfreauC Guthrie
Jan 31, 1998·The EMBO Journal·B DichtlD Tollervey
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·K Zhang, A W Nicholson

❮ Previous
Next ❯

Citations

Oct 28, 2010·Journal of Plant Research·Eri KiyotaToshiyuki Fukuhara
Mar 29, 2003·Journal of Molecular Biology·Bruno LamontagneSherif Abou Elela
Jun 17, 1999·FEMS Microbiology Reviews·A W Nicholson
Nov 30, 2002·Biochimie·Jean Pierre BachellerieAlexander Hüttenhofer
Jun 18, 2002·Current Opinion in Cell Biology·Witold Filipowicz, Vanda Pogacić
Jan 26, 2002·The International Journal of Biochemistry & Cell Biology·Christian Conrad, Reinhard Rauhut
Nov 27, 2010·Quarterly Reviews of Biophysics·Bo Liang, Hong Li
Jul 10, 2008·The Journal of Biological Chemistry·Dorota Piekna-PrzybylskaMaurille J Fournier
Apr 20, 2004·Molecular Biology of the Cell·Mathieu CatalaSherif Abou Elela
Dec 8, 1998·The EMBO Journal·A HenrasM Caizergues-Ferrer
Dec 24, 1998·Nucleic Acids Research·R L SeipeltB C Rymond
Jul 9, 2011·Nucleic Acids Research·Andrew H Babiskin, Christina D Smolke
Sep 22, 2011·Nucleic Acids Research·Mathieu LavoieSherif Abou Elela
Oct 25, 2011·Nucleic Acids Research·Defne E EgeciogluGuillaume F Chanfreau
May 12, 2012·Nucleic Acids Research·Mathieu CatalaSherif Abou Elela
Apr 1, 2008·Eukaryotic Cell·Héloïse MullerCécile Fairhead
Nov 10, 2007·Molecular and Cellular Biology·Mathieu CatalaSherif Abou Elela
Mar 25, 2000·Molecular and Cellular Biology·D L Lafontaine, D Tollervey
Mar 31, 2005·Molecular and Cellular Biology·Ghada GhazalSherif Abou Elela

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.