PMID: 3320051Dec 1, 1987Paper

Processing of a wheat light-harvesting chlorophyll a/b protein precursor by a soluble enzyme from higher plant chloroplasts

The Journal of Cell Biology
G K Lamppa, M S Abad

Abstract

A processing activity has been identified in higher plant chloroplasts that cleaves the precursor of the light-harvesting chlorophyll a/b-binding protein (LHCP). A wheat LHCP gene previously characterized (Lamppa, G.K., G. Morelli, and N.-H. Chua, 1985. Mol. Cell Biol. 5:1370-1378) was used to synthesize RNA and subsequently the labeled precursor polypeptide in vitro. Incubation of the LHCP precursors with a soluble extract from lysed chloroplasts, after removal of the thylakoids and membrane vesicles, resulted in the release of a single 25-kD peptide. In contrast, when the LHCP precursors were used in an import reaction with intact pea or wheat chloroplasts, two forms (25 and 26 kD) of mature LHCP were found. The peptide released by the processing activity in the organelle-free assay comigrated with the lower molecular mass form of mature LHCP produced during import. Properties of the processing activity suggest that it is an endopeptidase. Chloroplasts from both pea and wheat, two divergent higher plants, contain the processing enzyme, suggesting its physiological importance in LHCP assembly into the thylakoids. We discuss the implications of LHCP precursor processing by a soluble enzyme that may be in the stromal compartment.

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Citations

Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Richter, G K Lamppa
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·P S VanderVereG K Lamppa
Oct 14, 2003·Eukaryotic Cell·Einar J StauberMichael Hippler
Aug 16, 2008·Plant Molecular Biology·Ewa Firlej-KwokaBettina Bölter
Feb 19, 2002·The Plant Journal : for Cell and Molecular Biology·M HipplerP Hoerth
Jun 12, 1999·Journal of Photochemistry and Photobiology. B, Biology·C De LucaR Bassi
Oct 25, 2006·The Journal of Cell Biology·Ming Li, Danny J Schnell
Nov 26, 1990·European Journal of Biochemistry·J E ReedP V Viitanen
Aug 1, 1989·Current Opinion in Cell Biology·J C Gray
May 5, 2016·Molecular Plant·Bettina Bölter, Jürgen Soll
Jul 1, 1991·Journal of Basic and Clinical Physiology and Pharmacology·S YalovskyR Nechushtai
Jan 1, 1988·Photosynthesis Research·M L Mishkind, S E Scioli
Feb 8, 1994·Biochimica Et Biophysica Acta·S Jansson
Nov 13, 1991·Biochimica Et Biophysica Acta·A Douwe de Boer, P J Weisbeek

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