PMID: 6165585Apr 1, 1981Paper

Processing of naked 45-S ribosomal RNA precursor in vitro by an RNA-associated endoribonuclease

European Journal of Biochemistry
C DenoyaJ L La Torre

Abstract

A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was easily obtained using an RNA extraction procedure based on a mild deproteinization with Sarkosyl and cold phenol/chloroform. This technique assured the recovery of several proteins and the endoribonuclease in association with the RNA. It was demonstrated that this endoribonuclease was capable of promoting, in vitro, a precise processing of naked 45-S ribosomal RNA precursor to molecules resembling the intermediates as well as the 28-S and 18-S cytoplasmic RNAs found in vivo. The presence of magnesium ions was required for the correct processing function of the enzyme. In addition, under the same conditions, the mature ribosomal RNA substrates were degraded at a slower rate by this RNA-associated RNase. It was possible to fractionate the enzymatic preparation into two different populations by means of a sucrose gradient: one associated and the other partially free of an RNA component. The effect of the intrinsic RNA associated with the endoribonuclease on the enzymatic activity was tested by analyzing both the enzymatic populations and the total enzymatic preparation treated with pronase or with immobilized pancreatic RNase. In all cas...Continue Reading

References

May 15, 1977·Analytical Biochemistry·A L Rosenthal, S A Lacks
Jan 1, 1978·Archives of Virology·C D DenoyaJ L La Torre
Jan 1, 1979·Archives of Virology·E A ScodellerJ L La Torre
Jul 1, 1977·FEBS Letters·A KissP Venetianer
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A E DahlbergM Zahalak
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B C StarkS Altman
Oct 1, 1979·FEBS Letters·C D DenoyaJ L La Torre
Mar 30, 1978·Biochemical and Biophysical Research Communications·A B Bransgrove, C L Cosquer
Jan 1, 1976·Annual Review of Biochemistry·R P Perry
Jan 20, 1975·Biochemical and Biophysical Research Communications·T InoueY Umemura
Sep 7, 1973·Biochimica Et Biophysica Acta·A W PrestaykoH Busch
Mar 14, 1972·Biochimica Et Biophysica Acta·R P PerryJ R Greenberg
Aug 26, 1971·Biochimica Et Biophysica Acta·C A AgsteribbeC Aaij
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S FerrariE Dickson
Feb 1, 1972·FEBS Letters·Marc Edouard Mirault, Klaus Scherrer

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