PMID: 6990410Mar 1, 1980Paper

Processing of precursors of 21S ribosomal RNA from yeast mitochondria

Proceedings of the National Academy of Sciences of the United States of America
S MertenM Rabinowitz

Abstract

The transcription and processing of mitochondrial 21S rRNA in a petite strain of Saccharomyces cerevisiae has been examined by electron microscopic analysis of R-loop hybrids and by hybridization of labeled mitochondrial DNA probes to RNA transferred to diazobenzyloxymethyl paper. We have shown the presence of a large [5.1- to 5.4-kilobase (kb)] transcript that appears to be a precursor of mitochondrial 21S rRNA. This transcript contains sequences homologous to those of the mature 21S rRNA, to the intervening sequence present in the gene, and to additional sequences at the 3' end of the molecule. Our data suggest that this precursor of 21S rRNA is processed in two steps. The intron sequence is usually excised first, followed by removal of the extra 3' sequences. In some cases, however, the 3' extension is first removed and the intron sequence is then excised. Both pathways appear to lead to formation of the 3.1-kb mature 21S rRNA and a stable 1.2-kb intron transcript. Similar results were obtained with grande MH41-7B mitochondrial RNA by RNA transfer hybridization. We have also observed a number of additional transcripts that may be normal processing intermediates or may result from faulty cleavage-ligation during excision of t...Continue Reading

References

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Citations

Feb 1, 1980·Current Genetics·J LockerM Rabinowitz
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H NomiyamaY Takagi
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N C Martin, K Underbrink-Lyon
Feb 11, 1982·Nucleic Acids Research·M W BeilharzP Nagley
Jan 25, 1983·Nucleic Acids Research·F Sor, H Fukuhara
Oct 11, 1984·Nucleic Acids Research·C PalleschiL Frontali
Dec 11, 1981·Nucleic Acids Research·G S GrootH L Pijst
Dec 11, 1981·Nucleic Acids Research·P BoernerT D Fox
Jan 1, 1981·Annals of the New York Academy of Sciences·J LockerM Rabinowitz
Oct 1, 1979·Analytical Biochemistry·J Locker
Jun 2, 2015·Cell Cycle·Dasmanthie De SilvaAntoni Barrientos
Nov 12, 2013·Cell Metabolism·Dasmanthie De SilvaAntoni Barrientos
Jan 1, 1983·CRC Critical Reviews in Biochemistry·H F TabakP Borst
Feb 18, 2021·Nature Reviews. Molecular Cell Biology·Eva Kummer, Nenad Ban
Apr 1, 1982·Molecular and Cellular Biology·P D Yuckenberg, S L Phillips

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