Processing of recombination intermediates by the RuvABC proteins

Annual Review of Genetics
S C West

Abstract

The RuvA, RuvB, and RuvC proteins in Escherichia coli play important roles in the late stages of homologous genetic recombination and the recombinational repair of damaged DNA. Two proteins, RuvA and RuvB, form a complex that promotes ATP-dependent branch migration of Holliday junctions, a process that is important for the formation of heteroduplex DNA. Individual roles for each protein have been defined, with RuvA acting as a specificity factor that targets RuvB, the branch migration motor to the junction. Structural studies indicate that two RuvA tetramers sandwich the junction and hold it in an unfolded square-planar configuration. Hexameric rings of RuvB face each other across the junction and promote a novel dual helicase action that "pumps" DNA through the RuvAB complex, using the free energy provided by ATP hydrolysis. The third protein, RuvC endonuclease, resolves the Holliday junction by introducing nicks into two DNA strands. Genetic and biochemical studies indicate that branch migration and resolution are coupled by direct interactions between the three proteins, possibly by the formation of a RuvABC complex.

References

Jan 16, 1976·Molecular & General Genetics : MGG·K A Stacey, R G Lloyd
Oct 1, 1992·Molecular Microbiology·S C West, B Connolly
Oct 1, 1992·Molecular & General Genetics : MGG·I R TsanevaS C West
Oct 1, 1992·Current Opinion in Genetics & Development·R G Lloyd, G J Sharples
Jan 1, 1992·Annual Review of Biochemistry·S C West
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A ParsonsS C West
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B ConnollyS C West
Nov 1, 1991·Journal of Bacteriology·R G Lloyd, G J Sharples
Feb 1, 1991·Molecular & General Genetics : MGG·F BensonR G Lloyd
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B Connolly, S C West
Jan 1, 1990·Annual Review of Biochemistry·S W Matson, K A Kaiser-Rogers
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M LichtenA Nicolas
May 11, 1990·Nucleic Acids Research·E von KitzingS Diekmann
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P Cooper, P J Hagerman
Dec 20, 1987·Journal of Molecular Biology·J P Cooper, P J Hagerman
Oct 7, 1988·Cell·D R DuckettD M Lilley
Jan 1, 1987·Annual Review of Genetics·G Mosig
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M E ChurchillN C Seeman
Jan 1, 1974·Annual Review of Microbiology·R D Hotchkiss
Sep 1, 1967·The Journal of Experimental Medicine·L W Wannamaker, W Yasmineh
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M Lichten, M S Fox

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Citations

Oct 7, 2008·Functional & Integrative Genomics·William D BovillJason A Able
Aug 4, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Tatiana Garcia-Muse, Simon J Boulton
Nov 27, 2008·Cell Biochemistry and Biophysics·Magda Budzowska, Roland Kanaar
Dec 4, 2003·Journal of Molecular Biology·Ferenc OlaszJános Kiss
Apr 21, 2004·Journal of Molecular Biology·Dennis YoonJunghuei Chen
Oct 16, 2003·Trends in Biochemical Sciences·Wolf Dietrich HeyerJachen A Solinger
Nov 7, 2002·Journal of Molecular Biology·Ulrich Rass, Börries Kemper
Nov 13, 2002·Mutation Research·Leonard Wu, Ian D Hickson
Nov 5, 2003·Journal of Biochemical and Biophysical Methods·Mark J DickmanDavid P Hornby
Dec 21, 2000·Biochimica Et Biophysica Acta·S GonzalezJ G Wetmur
Jul 27, 2002·Trends in Genetics : TIG·Peter McGlynn, Robert G Lloyd
Dec 22, 1999·Mutation Research·J A Eisen, P C Hanawalt
Apr 8, 2000·Current Opinion in Genetics & Development·K J Marians
Feb 13, 2001·Trends in Biochemical Sciences·J Y Masson, S C West
Aug 11, 1998·Trends in Biochemical Sciences·P Baumann, S C West
Nov 1, 2002·Molecular Cell·Daniel L Kaplan, Mike O'Donnell
Oct 6, 2001·Current Opinion in Microbiology·K Kobryn, G Chaconas
Mar 2, 2011·Cell Research·Diana C Hargreaves, Gerald R Crabtree
Nov 3, 2004·Nature Reviews. Molecular Cell Biology·Yilun Liu, Stephen C West
Jun 17, 1998·Nature Structural Biology·D HargreavesJ B Rafferty
Jul 15, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K IshiokaH Shinagawa
Nov 13, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K IchiyanagiH Shinagawa
Oct 13, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M YoshikawaH Shinagawa
Mar 22, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y Nakatani
Nov 9, 2000·Molecular Microbiology·M SeigneurB Michel
Mar 5, 2002·European Journal of Biochemistry·Stuart M InglestonRobert G Lloyd
Nov 9, 2002·European Journal of Biochemistry·Mark J DickmanDavid P Hornby
Aug 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bénédicte MichelVladimir Bidnenko
Jul 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lu ZhaoZhaoxia Sun
Jan 24, 2012·The Journal of Biological Chemistry·Annamarie S CarterSteven W Matson
Apr 14, 2012·The Journal of Biological Chemistry·Avvaru N SuhasiniRobert M Brosh
Jun 19, 2001·The EMBO Journal·G ChaconasP Rosa

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Datasets Mentioned

BETA
Y08885
U38892

Methods Mentioned

BETA
footprinting
gel filtration
crosslinking studies
X-ray

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