Processing of the bovine spongiform encephalopathy-specific prion protein by dendritic cells.

Journal of Virology
Catherine Rybner-BarnierFrançoise Lazarini

Abstract

Dendritic cells (DC) are suspected to be involved in transmissible spongiform encephalopathies, including bovine spongiform encephalopathy (BSE). We detected the disease-specific, protease-resistant prion protein (PrP(bse)) in splenic DC purified by magnetic cell sorting 45 days after intraperitoneal inoculation of BSE prions in immunocompetent mice. We showed that bone marrow-derived DC (BMDC) from wild-type or PrP-null mice acquired both PrP(bse) and prion infectivity within 2 h of in vitro culture with a BSE inoculum. BMDC cleared PrP(bse) within 2 to 3 days of culture, while BMDC infectivity was only 10-fold diminished between days 1 and 6 of culture, suggesting that the infectious unit in BMDC is not removed at the same rate as PrP(bse) is removed from these cells. Bone marrow-derived plasmacytoid DC and bone marrow-derived macrophages (BMM) also acquired and degraded PrP(bse) when incubated with a BSE inoculum, with kinetics very similar to those of BMDC. PrP(bse) capture is probably specific to antigen-presenting cells since no uptake of PrP(bse) was observed when splenic B or T lymphocytes were incubated with a BSE inoculum in vitro. Lipopolysaccharide activation of BMDC or BMM prior to BSE infection resulted in an acce...Continue Reading

References

Jun 1, 1990·The Journal of Cell Biology·A TaraboulosS B Prusiner
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·J P DiSantoK Rajewsky
Jul 2, 1993·Cell·H BüelerC Weissmann
Dec 31, 1997·Nature·M A KleinA Aguzzi
Oct 21, 2000·Lancet·F HoustonC J Bostock
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·D ViletteH Laude
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·F MontrasioC Weissmann
Apr 3, 2001·Nature Medicine·M A KleinA Aguzzi
Sep 7, 2001·The Journal of Clinical Investigation·P AucouturierT Wisniewski
Dec 26, 2001·The Journal of General Virology·Fang-Ping HuangG Gordon MacPherson
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Marco PrinzAdriano Aguzzi
Oct 5, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Olivier AndréolettiFrançois Schelcher
Nov 5, 2002·Journal of Virology·Katarina M LuhrKrister Kristensson
Dec 6, 2002·The American Journal of Pathology·Oskar KoperekGeorg Wick
Mar 26, 2003·The American Journal of Pathology·Lynn M HerrmannKatherine I O'Rourke
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Víctor Gabriel MorónClaude Leclerc
Aug 11, 2004·Lancet·Alexander H PedenJames W Ironside
Sep 3, 2004·Nature Reviews. Immunology·Adriano Aguzzi, Christina J Sigurdson
Dec 14, 2004·Journal of Neuroimmunology·Nicole C KaneiderChristian J Wiedermann
Jan 22, 2005·Science·Mathias HeikenwalderAdriano Aguzzi
Jun 14, 2005·Journal of Neuroimmunology·Barbara RosicarelliFrancesca Aloisi

❮ Previous
Next ❯

Citations

Jul 12, 2007·Histochemistry and Cell Biology·Gauthier DorbanNadine Antoine
Mar 20, 2010·Histochemistry and Cell Biology·Gauthier DorbanNadine Antoine
Feb 24, 2012·PLoS Pathogens·Rocio Castro-SeoanePeter-Christian Klöhn
Jan 25, 2013·Viruses·Barry M Bradford, Neil A Mabbott
Aug 4, 2009·Veterinary Immunology and Immunopathology·Yukiko SassaNaotaka Ishiguro
May 23, 2008·Journal of Neuroimmunology·Grégory DoréFrançoise Lazarini
Dec 24, 2015·Journal of Immunology Research·Neil Andrew Mabbott, Barry Matthew Bradford
Oct 20, 2007·The FEBS Journal·Sabine GilchHermann M Schätzl
Jul 31, 2010·The Biochemical Journal·Christelle LangevinChiara Zurzolo
Sep 7, 2012·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Gorka Barrenetxea
Jan 7, 2017·The Journal of Experimental Medicine·Charlotte ViantNicholas D Huntington
Jan 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adriana Flores-LangaricaGordon G MacPherson
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gloria Martínez del HoyoPierre Aucouturier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bovine Spongiform Encephalopathy

Bovine spongiform encephalopathy is a neurodegenerative disease belonging to the transmissible spongiform encephalopathies, a group of diseases including sheep scrapie and human Creutzfeldt-Jakob disease. Here is the latest research.

Bovine Spongiform Encephalopathy (MDS)

Bovine spongiform encephalopathy is a neurodegenerative disease belonging to the transmissible spongiform encephalopathies, a group of diseases including sheep scrapie and human Creutzfeldt-Jakob disease. Here is the latest research.