Processing of the E1 glycoprotein of hepatitis C virus expressed in mammalian cells

The Journal of General Virology
A Fournillier JacobC Wychowski

Abstract

The structural part of the hepatitis C virus (HCV) genome encodes a capsid protein, C and two envelope glycoproteins, E1 and E2, released from the virus polyprotein precursor by signalase(s) cleavage(s). The processing of E1 was investigated by infecting simian cells with recombinant vaccinia viruses expressing parts of the HCV structural proteins. When the predicted E1 sequence was expressed alone (amino acid residues 174-370 of the polyprotein) or with the capsid protein gene (residues 1-370). it showed an apparent molecular mass of 35 kDa as measured by SDS-PAGE analysis. However, when E1 was expressed as part of a truncated C-E1-truncated E2 polypeptide (residues 132-383), the processed E1 product had the expected apparent molecular mass of 31 kDa, suggesting that flanking sequences are necessary for the generation of the mature 31 kDa El form. The N-terminal sequence of the two E1 forms was found to be the same. Analysis of the glycosylation pattern showed that, in both species, only four of the five potential N-linked glycosylation sites were recognized, indicating that glycosylation was not involved in the molecular mass difference. We showed that expression of E1 with or without the hydrophobic stretch of amino acids re...Continue Reading

Citations

Oct 1, 1996·Journal of Medical Virology·A Fournillier-JacobC Wychowski
Jun 27, 2002·Biochemical and Biophysical Research Communications·Nelson Acosta-RiveroJuan Morales
Jun 18, 2002·The EMBO Journal·Laurence CocquerelJean Dubuisson
Mar 22, 2002·Journal of Virology·Séverine Carrère-KremerJean Dubuisson
Jun 16, 2005·Journal of Virology·Anne GoffardJean Dubuisson
Feb 21, 2006·Biochemical and Biophysical Research Communications·Gillian Martinez-DonatoSantiago Dueñas-Carrera
Sep 8, 2011·Current Protocols in Pharmacology·Magdeleine HungXiaohong Liu
Jun 18, 2003·The Journal of General Virology·Juliette RousselCzeslaw Wychowski
May 29, 2004·The Journal of General Virology·Søren U NielsenGeoffrey L Toms
Sep 26, 2001·Proceedings. Biological Sciences·M P Stumpf, N Zitzmann
Jul 1, 1999·Protein Science : a Publication of the Protein Society·A Y HoweJ Y Lau
Jan 16, 2010·Journal of Gastroenterology and Hepatology·Sampa PalAditi Das
Nov 24, 1999·The Journal of General Virology·A ChoukhiJ Dubuisson

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