PMID: 2124700Dec 1, 1990Paper

Processing of the mother-cell sigma factor, sigma K, may depend on events occurring in the forespore during Bacillus subtilis development

Proceedings of the National Academy of Sciences of the United States of America
S LuL Kroos

Abstract

During sporulation of the Gram-positive bacterium Bacillus subtilis, transcription of genes encoding spore coat proteins in the mother-cell compartment of the sporangium is controlled by RNA polymerase containing the sigma subunit called sigma K. Based on comparison of the N-terminal amino acid sequence of sigma K with the nucleotide sequence of the gene encoding sigma K (sigK), the primary product of sigK was inferred to be a pro-protein (pro-sigma K) with 20 extra amino acids at the N terminus. Using antibodies generated against pro-sigma K, we have detected pro-sigma K beginning at the third hour of sporulation and sigma K beginning about 1 hr later. Even when pro-sigma K is expressed artificially during growth and throughout sporulation, sigma K appears at the normal time and expression of a sigma K-controlled gene occurs normally. These results suggest that pro-sigma K is an inactive precursor that is proteolytically processed to active sigma K in a developmentally regulated fashion. Mutations that block forespore gene expression block accumulation of sigma K but not accumulation of pro-sigma K, suggesting that pro-sigma K processing is a regulatory device that couples the programs of gene expression in the two compartment...Continue Reading

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Citations

Feb 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W Gober
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A Driks, R Losick
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D L Popham, P Stragier
Jan 24, 2012·Applied and Environmental Microbiology·Wasin Buasri, Watanalai Panbangred
Apr 16, 2013·Journal of Bacteriology·Ruanbao ZhouLee Kroos
Aug 23, 2006·Journal of Bacteriology·Alisa W SerioAbraham L Sonenshein
Jan 12, 2000·Journal of Bacteriology·D H Green, S M Cutting
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·David W Hilbert, Patrick J Piggot
Jan 1, 1996·Annual Review of Genetics·P Stragier, R Losick
Oct 8, 2013·PLoS Genetics·Fátima C PereiraAdriano O Henriques
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·D Z RudnerR Losick
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·O Resnekov, R Losick
Apr 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ruanbao Zhou, Lee Kroos
Jun 4, 1993·Cell·D Kaiser, R Losick
Nov 14, 2014·Molecular Microbiology·Keyan PishdadianAimee Shen
Dec 21, 2013·FEMS Microbiology Reviews·Anna KonovalovaLee Kroos
Sep 14, 2010·Current Biology : CB·Michiel J L de HoonDennis Vitkup
Dec 13, 2001·Developmental Cell·D Z Rudner, R Losick
Jan 27, 2000·Cell·L Shapiro, R Losick
Apr 1, 1993·Current Opinion in Genetics & Development·M A Strauch, J A Hoch
Jan 10, 1998·The Journal of Biological Chemistry·B D Johnson, A J Dombroski
Nov 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Sabyasachi HalderLee Kroos

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